<b>Concentration of anesthetic for long-term exposure and the effects of inter-suture distance in the goldfish <em>Carassius auratus</em></b> - DOI: 10.4025/actascibiolsci.v31i2.802 <b>Concentration of anesthetic for long-term exposure and the effects of inter-suture distance in the goldfish <em>Carassius auratus</em></b> - DOI: 10.4025/actascibiolsci.v31i2.802

• Main Authors: Eduardo Shimoda, George Shigueki Yasui, Anne Marie Yasui, Oswaldo Pinto Ribeiro-Filho
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2009-05-01
• Series: Acta Scientiarum : Biological Sciences
• Subjects: anesthetic; surgery; healing; fish; suture
• Online Access: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/802

This study aimed to determine the minimum concentration of 2-phenoxyethanol for long-term exposure and evaluate the effect of inter-suture distance on wound healing in the goldfish <em>Carassius auratus</em>. Twenty adult goldfish (standard length = 12.4 ± 1.1 cm; weight = 58.7 ± 17.2 g) were anesthetized in 2-phenoxiethanol at 1.2‰ and placed in an anesthesia delivery system at the following concentrations of 2-phenoxiethanol: 0.0 (control); 0.1; 0.2; 0.3 and 0.4‰, and the duration of sedation was measured. Fifteen days later, fishes were anesthetized using the same procedure, and a 36 mm incision was performed in the ventro-lateral region. The incision was sutured using a simple-interrupted pattern with 3, 6 or 9 mm as inter-suture distances. Results demonstrated that 2-phenoxiethanol at 0.4‰ maintain the sedation for surgical procedures up to 60 minutes, and 9 mm as inter-suture distance optimized the wound healing in goldfish.<br>This study aimed to determine the minimum concentration of 2-phenoxyethanol for long-term exposure and evaluate the effect of inter-suture distance on wound healing in the goldfish <em>Carassius auratus</em>. Twenty adult goldfish (standard length = 12.4 ± 1.1 cm; weight = 58.7 ± 17.2 g) were anesthetized in 2-phenoxiethanol at 1.2‰ and placed in an anesthesia delivery system at the following concentrations of 2-phenoxiethanol: 0.0 (control); 0.1; 0.2; 0.3 and 0.4‰, and the duration of sedation was measured. Fifteen days later, fishes were anesthetized using the same procedure, and a 36 mm incision was performed in the ventro-lateral region. The incision was sutured using a simple-interrupted pattern with 3, 6 or 9 mm as inter-suture distances. Results demonstrated that 2-phenoxiethanol at 0.4‰ maintain the sedation for surgical procedures up to 60 minutes, and 9 mm as inter-suture distance optimized the wound healing in goldfish.