Low frequency of p53 mutations in cervical carcinomas among Brazilian women
Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene prod...
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Associação Brasileira de Divulgação Científica
2001-06-01
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005 |
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doaj-5187999a45664ea780f56ef7fa5fe5c72020-11-24T22:40:44ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2001-06-0134672773310.1590/S0100-879X2001000600005Low frequency of p53 mutations in cervical carcinomas among Brazilian womenN.A. PinheiroL.L. VillaHuman papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation polymorphism analysis and DNA sequencing. Only four missense point mutations were detected. These findings suggest that other mechanisms independent of p53 inactivation may play a role in the genesis of cervical carcinomas.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005single-stranded conformation polymorphismSSCPsuppressor geneHPVPCRDNA sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
N.A. Pinheiro L.L. Villa |
spellingShingle |
N.A. Pinheiro L.L. Villa Low frequency of p53 mutations in cervical carcinomas among Brazilian women Brazilian Journal of Medical and Biological Research single-stranded conformation polymorphism SSCP suppressor gene HPV PCR DNA sequencing |
author_facet |
N.A. Pinheiro L.L. Villa |
author_sort |
N.A. Pinheiro |
title |
Low frequency of p53 mutations in cervical carcinomas among Brazilian women |
title_short |
Low frequency of p53 mutations in cervical carcinomas among Brazilian women |
title_full |
Low frequency of p53 mutations in cervical carcinomas among Brazilian women |
title_fullStr |
Low frequency of p53 mutations in cervical carcinomas among Brazilian women |
title_full_unstemmed |
Low frequency of p53 mutations in cervical carcinomas among Brazilian women |
title_sort |
low frequency of p53 mutations in cervical carcinomas among brazilian women |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2001-06-01 |
description |
Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation polymorphism analysis and DNA sequencing. Only four missense point mutations were detected. These findings suggest that other mechanisms independent of p53 inactivation may play a role in the genesis of cervical carcinomas. |
topic |
single-stranded conformation polymorphism SSCP suppressor gene HPV PCR DNA sequencing |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005 |
work_keys_str_mv |
AT napinheiro lowfrequencyofp53mutationsincervicalcarcinomasamongbrazilianwomen AT llvilla lowfrequencyofp53mutationsincervicalcarcinomasamongbrazilianwomen |
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