Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract
Abstract In the present report, three different shapes of chitosan-capped gold nanoparticles (nanospheres, nanostars, and nanorods) were synthesized to investigate the effects of shape on cytotoxicity and cellular uptake in cancer cells. Green tea extract was utilized as a reducing agent to reduce g...
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doaj-51891889726d46a88ad1c3e512edbc192020-11-25T03:54:37ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2019-04-0114111410.1186/s11671-019-2967-1Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extractYou Jeong Lee0Eun-Young Ahn1Youmie Park2College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje UniversityCollege of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje UniversityCollege of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje UniversityAbstract In the present report, three different shapes of chitosan-capped gold nanoparticles (nanospheres, nanostars, and nanorods) were synthesized to investigate the effects of shape on cytotoxicity and cellular uptake in cancer cells. Green tea extract was utilized as a reducing agent to reduce gold salts to gold nanospheres. Gold nanostars were prepared using an as-prepared nanosphere solution as a seed solution. Gold nanorods were synthesized using a conventional method. All three types of gold nanoparticles showed their characteristic surface plasmon resonance bands upon UV-visible spectrophotometry. In high-resolution transmission electron microscopy images, lattice structures were clearly observed in all three shapes, confirming the crystalline nature of the nanoparticles. All three colloidal solutions of gold nanoparticles retained colloidal stability in various solutions. To assess cytotoxicity, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed on four cancer cell lines. The cytotoxicity was the highest in nanorods, followed by nanostars and finally nanospheres. The cellular uptake of gold nanoparticles in human hepatocyte carcinoma cells (HepG2) was measured, and the results followed the order nanospheres > nanorods > nanostars. The outcomes of the current study may assist in the shape design of gold nanoparticles for therapeutic applications as drug delivery vehicles in the field of nanomedicine.http://link.springer.com/article/10.1186/s11671-019-2967-1Gold nanoparticlesGreen tea extractNanospheresNanostarsNanorodsCytotoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
You Jeong Lee Eun-Young Ahn Youmie Park |
spellingShingle |
You Jeong Lee Eun-Young Ahn Youmie Park Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract Nanoscale Research Letters Gold nanoparticles Green tea extract Nanospheres Nanostars Nanorods Cytotoxicity |
author_facet |
You Jeong Lee Eun-Young Ahn Youmie Park |
author_sort |
You Jeong Lee |
title |
Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract |
title_short |
Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract |
title_full |
Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract |
title_fullStr |
Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract |
title_full_unstemmed |
Shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract |
title_sort |
shape-dependent cytotoxicity and cellular uptake of gold nanoparticles synthesized using green tea extract |
publisher |
SpringerOpen |
series |
Nanoscale Research Letters |
issn |
1931-7573 1556-276X |
publishDate |
2019-04-01 |
description |
Abstract In the present report, three different shapes of chitosan-capped gold nanoparticles (nanospheres, nanostars, and nanorods) were synthesized to investigate the effects of shape on cytotoxicity and cellular uptake in cancer cells. Green tea extract was utilized as a reducing agent to reduce gold salts to gold nanospheres. Gold nanostars were prepared using an as-prepared nanosphere solution as a seed solution. Gold nanorods were synthesized using a conventional method. All three types of gold nanoparticles showed their characteristic surface plasmon resonance bands upon UV-visible spectrophotometry. In high-resolution transmission electron microscopy images, lattice structures were clearly observed in all three shapes, confirming the crystalline nature of the nanoparticles. All three colloidal solutions of gold nanoparticles retained colloidal stability in various solutions. To assess cytotoxicity, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed on four cancer cell lines. The cytotoxicity was the highest in nanorods, followed by nanostars and finally nanospheres. The cellular uptake of gold nanoparticles in human hepatocyte carcinoma cells (HepG2) was measured, and the results followed the order nanospheres > nanorods > nanostars. The outcomes of the current study may assist in the shape design of gold nanoparticles for therapeutic applications as drug delivery vehicles in the field of nanomedicine. |
topic |
Gold nanoparticles Green tea extract Nanospheres Nanostars Nanorods Cytotoxicity |
url |
http://link.springer.com/article/10.1186/s11671-019-2967-1 |
work_keys_str_mv |
AT youjeonglee shapedependentcytotoxicityandcellularuptakeofgoldnanoparticlessynthesizedusinggreenteaextract AT eunyoungahn shapedependentcytotoxicityandcellularuptakeofgoldnanoparticlessynthesizedusinggreenteaextract AT youmiepark shapedependentcytotoxicityandcellularuptakeofgoldnanoparticlessynthesizedusinggreenteaextract |
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