Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation
S. cerevisiae TBP associated factor 14 (Taf14) is a transcriptional regulator that interacts with multiple nuclear complexes. Here, the authors report that the extra-terminal domain of Taf14 (Taf14ET) recognizes a common motif in various transcriptional coactivator proteins and they solve the NMR st...
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Nature Publishing Group
2020-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-18021-7 |
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doaj-5199bb852e6d4eb29c3b0d7f59cc88812021-08-22T11:41:51ZengNature Publishing GroupNature Communications2041-17232020-08-0111111410.1038/s41467-020-18021-7Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separationGuochao Chen0Duo Wang1Bin Wu2Fuxiang Yan3Hongjuan Xue4Quanmeng Wang5Shu Quan6Yong Chen7State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of SciencesState Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of SciencesNational Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of SciencesState Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of SciencesNational Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of SciencesNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of SciencesS. cerevisiae TBP associated factor 14 (Taf14) is a transcriptional regulator that interacts with multiple nuclear complexes. Here, the authors report that the extra-terminal domain of Taf14 (Taf14ET) recognizes a common motif in various transcriptional coactivator proteins and they solve the NMR structure of Taf14ET bound the ET-binding motif of Sth1, the catalytic subunit of the RSC (Remodel the Structure of Chromatin) complex, and furthermore show that Taf14ET undergoes liquid-liquid phase separation, which is enhanced by Taf14 interaction partners.https://doi.org/10.1038/s41467-020-18021-7 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Guochao Chen Duo Wang Bin Wu Fuxiang Yan Hongjuan Xue Quanmeng Wang Shu Quan Yong Chen |
| spellingShingle |
Guochao Chen Duo Wang Bin Wu Fuxiang Yan Hongjuan Xue Quanmeng Wang Shu Quan Yong Chen Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation Nature Communications |
| author_facet |
Guochao Chen Duo Wang Bin Wu Fuxiang Yan Hongjuan Xue Quanmeng Wang Shu Quan Yong Chen |
| author_sort |
Guochao Chen |
| title |
Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation |
| title_short |
Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation |
| title_full |
Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation |
| title_fullStr |
Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation |
| title_full_unstemmed |
Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation |
| title_sort |
taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2020-08-01 |
| description |
S. cerevisiae TBP associated factor 14 (Taf14) is a transcriptional regulator that interacts with multiple nuclear complexes. Here, the authors report that the extra-terminal domain of Taf14 (Taf14ET) recognizes a common motif in various transcriptional coactivator proteins and they solve the NMR structure of Taf14ET bound the ET-binding motif of Sth1, the catalytic subunit of the RSC (Remodel the Structure of Chromatin) complex, and furthermore show that Taf14ET undergoes liquid-liquid phase separation, which is enhanced by Taf14 interaction partners. |
| url |
https://doi.org/10.1038/s41467-020-18021-7 |
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