Optimization of a Recombinant Lectin Production in <i>Pichia pastoris</i> Using Crude Glycerol in a Fed-Batch System

Optimization of a Recombinant Lectin Production in <i>Pichia pastoris</i> Using Crude Glycerol in a Fed-Batch System

The production of heterologous proteins for medical use is an important area of interest. The optimization of the bioprocesses includes the improvement of time, costs, and unit operations. Our study shows that a lectin fraction from Tepary bean (<i>Phaseolus acutifolius</i>) (TBLF) has c...

Full description

Bibliographic Details
Main Authors:	Dulce Palmerín-Carreño, Dania Martínez-Alarcón, José Luis Dena-Beltrán, Lineth Juliana Vega-Rojas, Alejandro Blanco-Labra, Antonio Escobedo-Reyes, Teresa García-Gasca
Format:	Article
Language:	English
Published:	MDPI AG 2021-05-01
Series:	Processes
Subjects:	crude glycerol optimization process fed-batch recombinant lectin rTBL-1 <i>Phaseolus acutifolius</i>
Online Access:	https://www.mdpi.com/2227-9717/9/5/876

Similar Items

Tepary Bean (<i>Phaseolus acutifolius</i>) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells
by: Ulisses Moreno-Celis, et al.
Published: (2020-02-01)

Tolerability assessment of a lectin fraction from Tepary bean seeds (Phaseolus acutifolius) orally administered to rats
by: Roberto Ferriz-Martínez, et al.
Published: (2015-01-01)

Phaseolus acutifolius Lectin Fractions Exhibit Apoptotic Effects on Colon Cancer: Preclinical Studies Using Dimethilhydrazine or Azoxi-Methane as Cancer Induction Agents
by: Ulisses Moreno-Celis, et al.
Published: (2017-10-01)

Phytochemical Characterization and Bioactivity of <i>Asparagus acutifolius</i>: A Focus on Antioxidant, Cytotoxic, Lipase Inhibitory and Antimicrobial Activities
by: Amel Hamdi, et al.
Published: (2021-06-01)

Recombinant Lectin from Tepary Bean (<i>Phaseolus acutifolius</i>) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification
by: Dania Martínez-Alarcón, et al.
Published: (2020-04-01)

Lectin-Mediated Binding of Engineered <i>Lactococcus lactis</i> to Cancer Cells
by: Tina Vida Plavec, et al.
Published: (2021-01-01)

Purification, Biochemical Characterization, and Bioactive Properties of a Lectin Purified from the Seeds of White Tepary Bean (Phaseolus Acutifolius Variety Latifolius)
by: Marco A. Becerril-Flores, et al.
Published: (2011-03-01)

Expression of Lectins in Heterologous Systems
by: Dania Martínez-Alarcón, et al.
Published: (2018-02-01)

Lectin-Mediated Bacterial Modulation by the Intestinal Nematode <i>Ascaris suum</i>
by: Ankur Midha, et al.
Published: (2021-08-01)

Optimisation of Recombinant Myrosinase Production in <i>Pichia pastoris</i>
by: Zuzana Rosenbergová, et al.
Published: (2021-04-01)

<i>N</i>-Acetyl-D-Glucosamine-Binding Lectin in <i>Acropora tenuis</i> Attracts Specific Symbiodiniaceae Cell Culture Strains
by: Ryota Takeuchi, et al.
Published: (2021-03-01)

Identification of 35 C-Type Lectins in the Oriental Armyworm, <i>Mythimna separata</i> (Walker)
by: Hao Li, et al.
Published: (2021-06-01)

Characterization of a Novel Mannose-Binding Lectin with Antiviral Activities from Red Alga, <i>Grateloupia chiangii</i>
by: Hyun-Ju Hwang, et al.
Published: (2020-02-01)

Oncolytic Vaccinia Virus Expressing <i>Aphrocallistes vastus</i> Lectin as a Cancer Therapeutic Agent
by: Tao Wu, et al.
Published: (2019-06-01)

Impedimetric Biosensor Based on a <i>Hechtia argentea</i> Lectin for the Detection of <i>Salmonella</i> spp.
by: Jorge Lopez-Tellez, et al.
Published: (2020-11-01)

Identification of <i>Echinacea Purpurea</i> (L.) Moench Root LysM Lectin with Nephrotoxic Properties
by: Gabriele Balciunaite, et al.
Published: (2020-01-01)

Banana Lectin from <i>Musa paradisiaca</i> Is Mitogenic for Cow and Pig PBMC via IL-2 Pathway and ELF1
by: Roxane L. Degroote, et al.
Published: (2021-08-01)

NUTRITIONAL QUALITY AND ORGANOLEPTIC ACCEPTABILITY OF AKARA PREPARED FROM GERMINATED TEPARY BEANS
by: Idouraine, Ahmed, 1948-
Published: (1987)

Induction of Recombinant Lectin Expression by an Artificially Constructed Tandem Repeat Structure: A Case Study Using <i>Bryopsis plumosa</i> Mannose-Binding Lectin
by: Hyun-Ju Hwang, et al.
Published: (2018-11-01)

Transiently Expressed Mistletoe Lectin II in <i>Nicotiana benthamiana</i> Demonstrates Anticancer Activity In Vitro
by: Milena Mazalovska, et al.
Published: (2020-05-01)

Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from <i>Pseudomonas aeruginosa</i>
by: Tünde Zita Illyés, et al.
Published: (2021-01-01)

Biological Activities of Wild Asparagus (Asparagus acutifolius L.)
by: Arzu Kaska, et al.
Published: (2018-09-01)

Man-Specific, GalNAc/T/Tn-Specific and Neu5Ac-Specific Seaweed Lectins as Glycan Probes for the SARS-CoV-2 (COVID-19) Coronavirus
by: Annick Barre, et al.
Published: (2020-10-01)

Produção heteróloga de frutalina em Pichia pastoris
by: Felix, Wagner Pereira
Published: (2016)

Improved microscale cultivation of Pichia pastoris for clonal screening
by: Alexander Eck, et al.
Published: (2018-05-01)

Expression of <i>Pleurotus ostreatus</i> Laccase Gene in <i>Pichia pastoris</i> and Its Degradation of Corn Stover Lignin
by: Qian Song, et al.
Published: (2020-04-01)

Expression and Characterization of an Alginate Lyase and Its Thermostable Mutant in <i>Pichia pastoris</i>
by: Suxiao Yang, et al.
Published: (2020-06-01)

Synthesis of β-d-galactopyranoside-Presenting Glycoclusters, Investigation of Their Interactions with <i>Pseudomonas aeruginosa</i> Lectin A (PA-IL) and Evaluation of Their Anti-Adhesion Potential
by: Lenka Malinovská, et al.
Published: (2019-11-01)

Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in <i>Pichia pastoris</i> for Biomedical Applications
by: Pia Gellermann, et al.
Published: (2019-09-01)

The T/Tn-Specific <i>Helix pomatia</i> Lectin Induces Cell Death in Lymphoma Cells Negative for T/Tn Antigens
by: Mathias Simplicien, et al.
Published: (2021-08-01)

Construction of a Lectin–Glycan Interaction Network from Enterohemorrhagic <i>Escherichia coli</i> Strains by Multi-omics Analysis
by: Seung-Hak Cho, et al.
Published: (2020-04-01)

High-Level Production of a Thermostable Mutant of <i>Yarrowia lipolytica</i> Lipase 2 in <i>Pichia pastoris</i>
by: Qinghua Zhou, et al.
Published: (2019-12-01)

Efficacy of Live Attenuated Vaccine and Commercially Available Lectin Against Avian Pathogenic <i>E. coli </i>Infection in Broiler Chickens
by: Ahmed I. Abd El-Mawgoud, et al.
Published: (2020-05-01)

Protein Concentrates on Tepary Bean (Phaseolus acutifolius Gray) as a Functional Ingredient: In silico Docking of Tepary Bean Lectin to Peroxisome Proliferator-Activated Receptor Gamma
by: Cristina López-Ibarra, et al.
Published: (2021-05-01)

<i>Entamoeba histolytica</i> Trophozoites Interact with the c-Met Receptor at the Surface of Liver Origin Cells through the Gal/GalNAc Amoebic Lectin
by: Jesus Pérez-Hernández, et al.
Published: (2021-09-01)

The B Subunit of PirAB<i><sup>vp</sup></i> Toxin Secreted from <i>Vibrio parahaemolyticus</i> Causing AHPND Is an Amino Sugar Specific Lectin
by: Marcelo Victorio-De Los Santos, et al.
Published: (2020-03-01)

Genome-Wide Characterization of Lectin Receptor Kinases in <i>Saccharum spontaneum</i> L. and Their Responses to <i>Stagonospora tainanensis</i> Infection
by: Zhoutao Wang, et al.
Published: (2021-02-01)

<i>Pichia pastoris</i> (<i>Komagataella phaffii</i>) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs)
by: Salomé de Sá Magalhães, et al.
Published: (2021-08-01)

Evaluation on Elongation Factor 1 Alpha of <i>Entamoeba histolytica</i> Interaction with the Intermediate Subunit of the Gal/GalNAc Lectin and Actin in Phagocytosis
by: Hang Zhou, et al.
Published: (2020-08-01)

Plant lectins, chemical and biological aspects
by: Renato de Azevedo Moreira, et al.
Published: (1991-01-01)