Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas

• Main Author: Michael A. Meyer
• Format: Article
• Language: English
• Published: MDPI AG 2014-06-01
• Series: Neurology International
• Subjects: brain tumor, glioma, gene, atlas
• Online Access: http://www.pagepress.org/journals/index.php/ni/article/view/5348

Gene expression within human glioblastomas were analyzed from data on 20,083 genes entered into the on-line Human Protein Atlas. In selecting genes that are strongly expressed within normal human brain tissue, 58 genes were identified from a search of the 20,083 entries that were rated as showing 90% or greater intensity of expression within normal brain tissues. Of these 58, a subset of 48 genes was identified that not only had expression data for human glioblastomas but also for the human glioblastoma cell line U-251. Four of these 48 selected genes were found to be strongly expressed within the cytoplasm when assessed by both histologic sampling of high grade glioma patient cases as well as U-251 glioblastoma cell line immunofluoresence analysis. These four human genes are: <em>AGBL2</em> (ATP/GTP binding protein-like 2), <em>BLOC1S6</em> (biogenesis of lysosomal organelles complex-1, subunit 6), <em>MAP1A</em> (microtubule-associated protein 1A) and <em>ZSWIM5</em> (zinc finger, SWIM-type containing 5, also known as <em>KIAA1511</em>). Further research is advocated to investigate the role of <em>ZSWIM5</em> and <em>AGBL2</em> in glioma cell biology.