Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas
Gene expression within human glioblastomas were analyzed from data on 20,083 genes entered into the on-line Human Protein Atlas. In selecting genes that are strongly expressed within normal human brain tissue, 58 genes were identified from a search of the 20,083 entries that were rated as showing 90...
Main Author: | |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2014-06-01
|
Series: | Neurology International |
Subjects: | |
Online Access: | http://www.pagepress.org/journals/index.php/ni/article/view/5348 |
id |
doaj-51bee09fbd2d40fd81e6c83ef0b56ee5 |
---|---|
record_format |
Article |
spelling |
doaj-51bee09fbd2d40fd81e6c83ef0b56ee52021-01-02T13:10:50ZengMDPI AGNeurology International2035-83852035-83772014-06-016210.4081/ni.2014.53482837Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein AtlasMichael A. Meyer0Department of Neurology, Sisters Hospital, Buffalo, NYGene expression within human glioblastomas were analyzed from data on 20,083 genes entered into the on-line Human Protein Atlas. In selecting genes that are strongly expressed within normal human brain tissue, 58 genes were identified from a search of the 20,083 entries that were rated as showing 90% or greater intensity of expression within normal brain tissues. Of these 58, a subset of 48 genes was identified that not only had expression data for human glioblastomas but also for the human glioblastoma cell line U-251. Four of these 48 selected genes were found to be strongly expressed within the cytoplasm when assessed by both histologic sampling of high grade glioma patient cases as well as U-251 glioblastoma cell line immunofluoresence analysis. These four human genes are: <em>AGBL2</em> (ATP/GTP binding protein-like 2), <em>BLOC1S6</em> (biogenesis of lysosomal organelles complex-1, subunit 6), <em>MAP1A</em> (microtubule-associated protein 1A) and <em>ZSWIM5</em> (zinc finger, SWIM-type containing 5, also known as <em>KIAA1511</em>). Further research is advocated to investigate the role of <em>ZSWIM5</em> and <em>AGBL2</em> in glioma cell biology.http://www.pagepress.org/journals/index.php/ni/article/view/5348brain tumor, glioma, gene, atlas |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael A. Meyer |
spellingShingle |
Michael A. Meyer Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas Neurology International brain tumor, glioma, gene, atlas |
author_facet |
Michael A. Meyer |
author_sort |
Michael A. Meyer |
title |
Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas |
title_short |
Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas |
title_full |
Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas |
title_fullStr |
Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas |
title_full_unstemmed |
Highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the Human Protein Atlas |
title_sort |
highly expressed genes in human high grade gliomas: immunohistochemical analysis of data from the human protein atlas |
publisher |
MDPI AG |
series |
Neurology International |
issn |
2035-8385 2035-8377 |
publishDate |
2014-06-01 |
description |
Gene expression within human glioblastomas were analyzed from data on 20,083 genes entered into the on-line Human Protein Atlas. In selecting genes that are strongly expressed within normal human brain tissue, 58 genes were identified from a search of the 20,083 entries that were rated as showing 90% or greater intensity of expression within normal brain tissues. Of these 58, a subset of 48 genes was identified that not only had expression data for human glioblastomas but also for the human glioblastoma cell line U-251. Four of these 48 selected genes were found to be strongly expressed within the cytoplasm when assessed by both histologic sampling of high grade glioma patient cases as well as U-251 glioblastoma cell line immunofluoresence analysis. These four human genes are: <em>AGBL2</em> (ATP/GTP binding protein-like 2), <em>BLOC1S6</em> (biogenesis of lysosomal organelles complex-1, subunit 6), <em>MAP1A</em> (microtubule-associated protein 1A) and <em>ZSWIM5</em> (zinc finger, SWIM-type containing 5, also known as <em>KIAA1511</em>). Further research is advocated to investigate the role of <em>ZSWIM5</em> and <em>AGBL2</em> in glioma cell biology. |
topic |
brain tumor, glioma, gene, atlas |
url |
http://www.pagepress.org/journals/index.php/ni/article/view/5348 |
work_keys_str_mv |
AT michaelameyer highlyexpressedgenesinhumanhighgradegliomasimmunohistochemicalanalysisofdatafromthehumanproteinatlas |
_version_ |
1724354045346316288 |