New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations
A simple, accurate, and precise spectrophotometric method has been proposed for the determination of three cephalosporins, namely: cefixime (cefi), cephalexine, (ceph) and cefotaxime (cefo) in pharmaceutical formulations. Proposed method is based on the derivatization of cephalosporins with 1,2-naph...
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doaj-51d4d61c04f245bb8bb0f280f92e3af92020-11-24T22:29:40ZengElsevierArabian Journal of Chemistry1878-53522015-03-018223323910.1016/j.arabjc.2011.08.012New spectrophotometric method for determination of cephalosporins in pharmaceutical formulationsShazalia M. Ali Ahmed0Abdalla A. Elbashir1Hassan Y. Aboul-Enein2University of Khartoum, Faculty of Science, Chemistry Department, Khartoum, SudanUniversity of Khartoum, Faculty of Science, Chemistry Department, Khartoum, SudanPharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, Cairo 12311, EgyptA simple, accurate, and precise spectrophotometric method has been proposed for the determination of three cephalosporins, namely: cefixime (cefi), cephalexine, (ceph) and cefotaxime (cefo) in pharmaceutical formulations. Proposed method is based on the derivatization of cephalosporins with 1,2-naphthoquinone-4-sulfonic (NQS). The optimum experimental conditions have been studied. Beer’s law is obeyed over the concentration of 0.5–3, 0.8–2.8, and 0.2–1.2 μg/mL for cefi, ceph, and cefo, respectively. The detection limits were 0.12, 0.168, and 0.0465 μg/mL for cefi, ceph, and cefo, respectively, with a linear regression correlation coefficient of 0.9993, 0.9993, and 0.9994 and recovery in range from 96.5–102.3, 96.04–102.22, and 97.09–99.3 for cefi, ceph, and cefo, respectively. Effects of pH, temperature, reaction time, and NQS concentration on the determination of cefi, ceph, and cefo, have been examined. This method is simple and can be applied for the determination of cefi, ceph, and cefo in pharmaceutical formulations in quality control laboratories.http://www.sciencedirect.com/science/article/pii/S1878535211002255Cephalosporins1,2-Naphthoquinone-4-sulfonic (NQS)Spectrophotometric analysisPharmaceutical analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shazalia M. Ali Ahmed Abdalla A. Elbashir Hassan Y. Aboul-Enein |
spellingShingle |
Shazalia M. Ali Ahmed Abdalla A. Elbashir Hassan Y. Aboul-Enein New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations Arabian Journal of Chemistry Cephalosporins 1,2-Naphthoquinone-4-sulfonic (NQS) Spectrophotometric analysis Pharmaceutical analysis |
author_facet |
Shazalia M. Ali Ahmed Abdalla A. Elbashir Hassan Y. Aboul-Enein |
author_sort |
Shazalia M. Ali Ahmed |
title |
New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations |
title_short |
New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations |
title_full |
New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations |
title_fullStr |
New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations |
title_full_unstemmed |
New spectrophotometric method for determination of cephalosporins in pharmaceutical formulations |
title_sort |
new spectrophotometric method for determination of cephalosporins in pharmaceutical formulations |
publisher |
Elsevier |
series |
Arabian Journal of Chemistry |
issn |
1878-5352 |
publishDate |
2015-03-01 |
description |
A simple, accurate, and precise spectrophotometric method has been proposed for the determination of three cephalosporins, namely: cefixime (cefi), cephalexine, (ceph) and cefotaxime (cefo) in pharmaceutical formulations. Proposed method is based on the derivatization of cephalosporins with 1,2-naphthoquinone-4-sulfonic (NQS). The optimum experimental conditions have been studied. Beer’s law is obeyed over the concentration of 0.5–3, 0.8–2.8, and 0.2–1.2 μg/mL for cefi, ceph, and cefo, respectively.
The detection limits were 0.12, 0.168, and 0.0465 μg/mL for cefi, ceph, and cefo, respectively, with a linear regression correlation coefficient of 0.9993, 0.9993, and 0.9994 and recovery in range from 96.5–102.3, 96.04–102.22, and 97.09–99.3 for cefi, ceph, and cefo, respectively. Effects of pH, temperature, reaction time, and NQS concentration on the determination of cefi, ceph, and cefo, have been examined. This method is simple and can be applied for the determination of cefi, ceph, and cefo in pharmaceutical formulations in quality control laboratories. |
topic |
Cephalosporins 1,2-Naphthoquinone-4-sulfonic (NQS) Spectrophotometric analysis Pharmaceutical analysis |
url |
http://www.sciencedirect.com/science/article/pii/S1878535211002255 |
work_keys_str_mv |
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