Correlation of reactive oxygen species contents in myocardial tissue and lung tissue with tissue injury and apoptosis in the rat models with sepsis

• Main Author: Wan-Ping Liu
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2018-08-01
• Series: Journal of Hainan Medical University
• Subjects: Sepsis; Reactive oxygen species; Myocardial injury; Lung injury; Apoptosis
• Online Access: http://www.hnykdxxb.com/PDF/201808/01.pdf

Objective: To study the correlation of reactive oxygen species (ROS) contents in myocardial tissue and lung tissue with tissue injury and apoptosis in the rat models with sepsis. Methods: The SD male rats were selected as experimental animals and divided into two groups, CLIP group were established into sepsis models by cecal ligation and puncture and Sham group received Sham operation. After model establishment, the serum was collected to determine the contents of myocardial injury and lung injury markers, and the lung tissue and myocardial tissue were collected to determine the contents of ROS and MDA as well as the expression of apoptosis genes. Results: ROS and MDA contents as well as PD-1, PD-L1, TWEAK, Caspase-3 and Caspase-9 mRNA expression in myocardial tissue and lung tissue of CLIP group were significantly higher than those of Sham group, and serum cTnI, CK-MB, SP-A, CC16 and KL-6 contents were significantly higher than those of Sham group; ROS content in myocardial tissue was positively correlated with cTnI and CK-MB contents in serum as well as PD-1, PD-L1, TWEAK, Caspase-3 and Caspase-9 mRNA expression in myocardial tissue, and ROS content in lung tissue was positively correlated with SP-A, CC16 and KL-6 contents in serum as well as PD-1, PD-L1, TWEAK, Caspase-3 and Caspase-9 mRNA expression in lung tissue. Conclusion: The increased generation of ROS in myocardial tissue and lung tissue of rat models with sepsis can cause tissue damage and apoptosis.