Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum
Abstract Background Cotton (Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries, but its production is on the decline due to the effects of salinity. Soil salt-alkalization leads to damage in cotton growth and a decrease in yields. Hyperosmolality-gated ca...
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BMC
2019-07-01
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Series: | Journal of Cotton Research |
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Online Access: | http://link.springer.com/article/10.1186/s42397-019-0028-z |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiu YANG Yanchao XU Fangfang YANG Richard Odongo MAGWANGA Xiaoyan CAI Xingxing WANG Yuhong WANG Yuqing HOU Kunbo WANG Fang LIU Zhongli ZHOU |
spellingShingle |
Xiu YANG Yanchao XU Fangfang YANG Richard Odongo MAGWANGA Xiaoyan CAI Xingxing WANG Yuhong WANG Yuqing HOU Kunbo WANG Fang LIU Zhongli ZHOU Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum Journal of Cotton Research OSCA gene family Gossypium hirsutum VIGS Salt and dehydration stress |
author_facet |
Xiu YANG Yanchao XU Fangfang YANG Richard Odongo MAGWANGA Xiaoyan CAI Xingxing WANG Yuhong WANG Yuqing HOU Kunbo WANG Fang LIU Zhongli ZHOU |
author_sort |
Xiu YANG |
title |
Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum |
title_short |
Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum |
title_full |
Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum |
title_fullStr |
Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum |
title_full_unstemmed |
Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum |
title_sort |
genome-wide identification of osca gene family and their potential function in the regulation of dehydration and salt stress in gossypium hirsutum |
publisher |
BMC |
series |
Journal of Cotton Research |
issn |
2523-3254 |
publishDate |
2019-07-01 |
description |
Abstract Background Cotton (Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries, but its production is on the decline due to the effects of salinity. Soil salt-alkalization leads to damage in cotton growth and a decrease in yields. Hyperosmolality-gated calcium-permeable channels (OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration. Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants. However, the molecular nature of the corresponding calcium ion channels remains unclearly. In this research work, we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genome-wide identification, characterization and functional analysis of the significantly up-regulated OSCA gene, GhOSCA1.1 through reverse genetics. Result A total of 35, 21 and 22 OSCA genes were identified in G. hirsutum, G. arboreum, and G. raimondii genomes, respectively, and were classified into four different clades according to their gene structure and phylogenetic relationship. Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN1_7TM (PF02714) domain. Moreover, the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress. Furthermore, the knockdown of one of the highly up-regulated genes, Gh_OSCA1.1 showed that the virus-induced gene silenced (VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants. Conclusion This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton. These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes, thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions. |
topic |
OSCA gene family Gossypium hirsutum VIGS Salt and dehydration stress |
url |
http://link.springer.com/article/10.1186/s42397-019-0028-z |
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doaj-51e1554982b4484f99ca06b42b3711642020-11-25T03:31:56ZengBMCJournal of Cotton Research2523-32542019-07-012111810.1186/s42397-019-0028-zGenome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutumXiu YANG0Yanchao XU1Fangfang YANG2Richard Odongo MAGWANGA3Xiaoyan CAI4Xingxing WANG5Yuhong WANG6Yuqing HOU7Kunbo WANG8Fang LIU9Zhongli ZHOU10Institute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS) / Research Base in Anyang Institute of Technology, State Key Laboratory of Cotton BiologyAbstract Background Cotton (Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries, but its production is on the decline due to the effects of salinity. Soil salt-alkalization leads to damage in cotton growth and a decrease in yields. Hyperosmolality-gated calcium-permeable channels (OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium concentration. Hyperosmolality-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in plants. However, the molecular nature of the corresponding calcium ion channels remains unclearly. In this research work, we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genome-wide identification, characterization and functional analysis of the significantly up-regulated OSCA gene, GhOSCA1.1 through reverse genetics. Result A total of 35, 21 and 22 OSCA genes were identified in G. hirsutum, G. arboreum, and G. raimondii genomes, respectively, and were classified into four different clades according to their gene structure and phylogenetic relationship. Gene and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN1_7TM (PF02714) domain. Moreover, the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic stress. Furthermore, the knockdown of one of the highly up-regulated genes, Gh_OSCA1.1 showed that the virus-induced gene silenced (VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed plants. Conclusion This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in cotton. These results provide a new insight of defense responses in general and lay the foundation for further investigation of the molecular role played by the OSCA genes, thereby providing suitable approaches to improve crop performance under salinity and drought stress conditions.http://link.springer.com/article/10.1186/s42397-019-0028-zOSCA gene familyGossypium hirsutumVIGSSalt and dehydration stress |