No evidence for involvement of <it>SDHD </it>in neuroblastoma pathogenesis

• Main Authors: Roels Frank, Van Roy Nadine, Combaret Valérie, Laureys Geneviève, Nuyts Annick, Smet Jöel, Vandenbroecke Caroline, Hoebeeck Jasmien, Vandesompele Jo, De Preter Katleen, Van Coster Rudy, Praet Marleen, De Paepe Anne, Speleman Frank
• Format: Article
• Language: English
• Published: BMC 2004-08-01
• Series: BMC Cancer
• Online Access: http://www.biomedcentral.com/1471-2407/4/55

<p>Abstract</p> <p>Background</p> <p>Deletions in the long arm of chromosome 11 are observed in a subgroup of advanced stage neuroblastomas with poor outcome. The deleted region harbours the tumour suppressor gene <it>SDHD </it>that is frequently mutated in paraganglioma and pheochromocytoma, which are, like neuroblastoma, tumours originating from the neural crest. In this study, we sought for evidence for involvement of <it>SDHD </it>in neuroblastoma.</p> <p>Methods</p> <p><it>SDHD </it>was investigated on the genome, transcriptome and proteome level using mutation screening, methylation specific PCR, real-time quantitative PCR based homozygous deletion screening and mRNA expression profiling, immunoblotting, functional protein analysis and ultrastructural imaging of the mitochondria.</p> <p>Results</p> <p>Analysis at the genomic level of 67 tumour samples and 37 cell lines revealed at least 2 bona-fide mutations in cell lines without allelic loss at 11q23: a 4bp-deletion causing skip of exon 3 resulting in a premature stop codon in cell line N206, and a Y93C mutation in cell line NMB located in a region affected by germline <it>SDHD </it>mutations causing hereditary paraganglioma. No evidence for hypermethylation of the <it>SDHD </it>promotor region was observed, nor could we detect homozygous deletions. Interestingly, <it>SDHD </it>mRNA expression was significantly reduced in <it>SDHD </it>mutated cell lines and cell lines with 11q allelic loss as compared to both cell lines without 11q allelic loss and normal foetal neuroblast cells. However, protein analyses and assessment of mitochondrial morphology presently do not provide clues as to the possible effect of reduced <it>SDHD </it>expression on the neuroblastoma tumour phenotype.</p> <p>Conclusions</p> <p>Our study provides no indications for 2-hit involvement of <it>SDHD </it>in the pathogenesis of neuroblastoma. Also, although a haplo-insufficient mechanism for <it>SDHD </it>involvement in advanced stage neuroblastoma could be considered, the present data do not provide consistent evidence for this hypothesis.</p>