GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis
Abstract Background Drought stress has great negative effects on the plant growth and development. The tolerance of plants to such abiotic stress is triggered by complicated and multilayered signaling pathways to restore cellular homeostasis and to promote survival. The WRKY family is one of the lar...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-10-01
|
Series: | BMC Plant Biology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12870-021-03238-5 |
id |
doaj-51f8858378cf447e83c9c20114ed8de5 |
---|---|
record_format |
Article |
spelling |
doaj-51f8858378cf447e83c9c20114ed8de52021-10-10T11:08:49ZengBMCBMC Plant Biology1471-22292021-10-0121111310.1186/s12870-021-03238-5GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesisQin Hu0Chuanwei Ao1Xiaorui Wang2Yanfei Wu3Xuezhu Du4State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityAbstract Background Drought stress has great negative effects on the plant growth and development. The tolerance of plants to such abiotic stress is triggered by complicated and multilayered signaling pathways to restore cellular homeostasis and to promote survival. The WRKY family is one of the largest transcription factor families in higher plants, and has been well recognized for the roles in regulating plants tolerance to abiotic and biotic stress. However, little is known about how the WRKY genes regulate drought resistance in cotton. Results In this work, we identified the WRKY transcription factor GhWRKY1-like from upland cotton as a positive regulator of tolerance to drought that directly manipulates abscisic acid (ABA) biosynthesis. Overexpression of GhWRKY1-like in Arabidopsis constitutively activated ABA biosynthesis genes, signaling genes, responsive genes and drought related maker genes, and led to enhanced tolerance to drought. Further analysis has shown that GhWRKY1-like can interact with “W-box” cis-elements of the promoters of AtNCED2, AtNCED5, AtNCED6 and AtNCED9 which are essential enzymes for ABA biosynthesis, and promotes the expression of those target genes. Conclusions In summary, our findings suggest that GhWRKY1-like may act as a positive regulator in Arabidopsis tolerance to drought via directly interacting with the promoters of AtNCED2, AtNCED5, AtNCED6 and AtNCED9 to promote ABA biosynthesis.https://doi.org/10.1186/s12870-021-03238-5Drought toleranceABATranscriptional regulationWRKYNCED |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qin Hu Chuanwei Ao Xiaorui Wang Yanfei Wu Xuezhu Du |
spellingShingle |
Qin Hu Chuanwei Ao Xiaorui Wang Yanfei Wu Xuezhu Du GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis BMC Plant Biology Drought tolerance ABA Transcriptional regulation WRKY NCED |
author_facet |
Qin Hu Chuanwei Ao Xiaorui Wang Yanfei Wu Xuezhu Du |
author_sort |
Qin Hu |
title |
GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis |
title_short |
GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis |
title_full |
GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis |
title_fullStr |
GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis |
title_full_unstemmed |
GhWRKY1-like, a WRKY transcription factor, mediates drought tolerance in Arabidopsis via modulating ABA biosynthesis |
title_sort |
ghwrky1-like, a wrky transcription factor, mediates drought tolerance in arabidopsis via modulating aba biosynthesis |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2021-10-01 |
description |
Abstract Background Drought stress has great negative effects on the plant growth and development. The tolerance of plants to such abiotic stress is triggered by complicated and multilayered signaling pathways to restore cellular homeostasis and to promote survival. The WRKY family is one of the largest transcription factor families in higher plants, and has been well recognized for the roles in regulating plants tolerance to abiotic and biotic stress. However, little is known about how the WRKY genes regulate drought resistance in cotton. Results In this work, we identified the WRKY transcription factor GhWRKY1-like from upland cotton as a positive regulator of tolerance to drought that directly manipulates abscisic acid (ABA) biosynthesis. Overexpression of GhWRKY1-like in Arabidopsis constitutively activated ABA biosynthesis genes, signaling genes, responsive genes and drought related maker genes, and led to enhanced tolerance to drought. Further analysis has shown that GhWRKY1-like can interact with “W-box” cis-elements of the promoters of AtNCED2, AtNCED5, AtNCED6 and AtNCED9 which are essential enzymes for ABA biosynthesis, and promotes the expression of those target genes. Conclusions In summary, our findings suggest that GhWRKY1-like may act as a positive regulator in Arabidopsis tolerance to drought via directly interacting with the promoters of AtNCED2, AtNCED5, AtNCED6 and AtNCED9 to promote ABA biosynthesis. |
topic |
Drought tolerance ABA Transcriptional regulation WRKY NCED |
url |
https://doi.org/10.1186/s12870-021-03238-5 |
work_keys_str_mv |
AT qinhu ghwrky1likeawrkytranscriptionfactormediatesdroughttoleranceinarabidopsisviamodulatingababiosynthesis AT chuanweiao ghwrky1likeawrkytranscriptionfactormediatesdroughttoleranceinarabidopsisviamodulatingababiosynthesis AT xiaoruiwang ghwrky1likeawrkytranscriptionfactormediatesdroughttoleranceinarabidopsisviamodulatingababiosynthesis AT yanfeiwu ghwrky1likeawrkytranscriptionfactormediatesdroughttoleranceinarabidopsisviamodulatingababiosynthesis AT xuezhudu ghwrky1likeawrkytranscriptionfactormediatesdroughttoleranceinarabidopsisviamodulatingababiosynthesis |
_version_ |
1716829979548844032 |