Polymorphic microsatellites in the human bloodfluke, <it>Schistosoma japonicum</it>, identified using a genomic resource
<p>Abstract</p> <p>Re-emergence of schistosomiasis in regions of China where control programs have ceased requires development of molecular-genetic tools to track gene flow and assess genetic diversity of <it>Schistosoma </it>populations. We identified many microsatelli...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-02-01
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| Series: | Parasites & Vectors |
| Online Access: | http://www.parasitesandvectors.com/content/4/1/13 |
| Summary: | <p>Abstract</p> <p>Re-emergence of schistosomiasis in regions of China where control programs have ceased requires development of molecular-genetic tools to track gene flow and assess genetic diversity of <it>Schistosoma </it>populations. We identified many microsatellite loci in the draft genome of <it>Schistosoma japonicum </it>using defined search criteria and selected a subset for further analysis. From an initial panel of 50 loci, 20 new microsatellites were selected for eventual optimization and application to a panel of worms from endemic areas. All but one of the selected microsatellites contain simple tri-nucleotide repeats. Moderate to high levels of polymorphism were detected. Numbers of alleles ranged from 6 to 14 and observed heterozygosity was always >0.6. The loci reported here will facilitate high resolution population-genetic studies on schistosomes in re-emergent foci.</p> |
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| ISSN: | 1756-3305 |