Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox spp.: Quercus ilex L. subsp. ballota [Desf.] Samp.
As a preliminary step in the phosphoproteome analysis of germinating seeds (0, and 24 h after seed imbibition) and early grown seedlings (216 h after seed imbibition) from a non-orthodox spp. Quercus ilex, a multiplex (SYPRO-Ruby and Pro-Q DPS) staining of high-resolution 2-DE gels was used. By usin...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2015-08-01
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Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00620/full |