Multiplex staining of 2-DE gels for an initial phosphoproteome analysis of germinating seeds and early grown seedlings from a non-orthodox spp.: Quercus ilex L. subsp. ballota [Desf.] Samp.

As a preliminary step in the phosphoproteome analysis of germinating seeds (0, and 24 h after seed imbibition) and early grown seedlings (216 h after seed imbibition) from a non-orthodox spp. Quercus ilex, a multiplex (SYPRO-Ruby and Pro-Q DPS) staining of high-resolution 2-DE gels was used. By usin...

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Bibliographic Details
Main Authors: María Cristina Romero-Rodriguez, Nieves eAbril, Rosa eSánchez-Lucas, Jesus V eJorrin Novo
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-08-01
Series:Frontiers in Plant Science
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Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00620/full

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