Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells
Successful implantation of the embryo into the human receptive endometrium is substantial for the establishment of a healthy pregnancy. This study focusses on the role of Syndecan-1 at the embryo-maternal interface, the multitasking coreceptor influencing ligand concentration, release and receptor p...
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Online Access: | http://dx.doi.org/10.1155/2017/8379256 |
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doaj-522b8da8f2994f9184df74c7ab2ccdf92020-11-24T22:17:05ZengHindawi LimitedMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/83792568379256Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast CellsDunja Maria Baston-Buest0Olga Altergot-Ahmad1Sarah Jean Pour2Jan-Steffen Krüssel3Udo Rudolf Markert4Tanja Natascha Fehm5Alexandra Petra Bielfeld6Department of Gynaecology, Center for Reproductive Medicine (UniKiD), Medical Faculty, University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanyDepartment of Gynaecology, Center for Reproductive Medicine (UniKiD), Medical Faculty, University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanyDepartment of Gynaecology, Center for Reproductive Medicine (UniKiD), Medical Faculty, University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanyDepartment of Gynaecology, Center for Reproductive Medicine (UniKiD), Medical Faculty, University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanyDepartment of Obstetrics, Research Lab/Placenta Lab, Research Center, Jena University, Building No. F2, Am Klinikum 1, 07747 Jena, GermanyDepartment of Gynaecology, Medical Faculty, University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanyDepartment of Gynaecology, Center for Reproductive Medicine (UniKiD), Medical Faculty, University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanySuccessful implantation of the embryo into the human receptive endometrium is substantial for the establishment of a healthy pregnancy. This study focusses on the role of Syndecan-1 at the embryo-maternal interface, the multitasking coreceptor influencing ligand concentration, release and receptor presentation, and cellular morphology. CXC motif ligand 1, being involved in chemotaxis and angiogenesis during implantation, is of special interest as a ligand of Syndecan-1. Human endometrial stromal cells with and without Syndecan-1 knock-down were decidualized and treated with specific inhibitors to evaluate signaling pathways regulating CXC ligand 1 expression. Western blot analyses of MAPK and Wnt members were performed, followed by analysis of spheroid interactions between human endometrial cells and extravillous trophoblast cells. By mimicking embryo contact using IL-1β, we showed less ERK and c-Jun activation by depletion of Syndecan-1 and less Frizzled 4 production as part of the canonical Wnt pathway. Additionally, more beta-catenin was phosphorylated and therefore degraded after depletion of Syndecan-1. Secretion of CXC motif ligand 1 depends on MEK-1 with respect to Syndecan-1. Regarding the interaction of endometrial and trophoblast cells, the spheroid center-to-center distances were smaller after depletion of Syndecan-1. Therefore, Syndecan-1 seems to affect signaling processes relevant to signaling and intercellular interaction at the trophoblast-decidual interface.http://dx.doi.org/10.1155/2017/8379256 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dunja Maria Baston-Buest Olga Altergot-Ahmad Sarah Jean Pour Jan-Steffen Krüssel Udo Rudolf Markert Tanja Natascha Fehm Alexandra Petra Bielfeld |
spellingShingle |
Dunja Maria Baston-Buest Olga Altergot-Ahmad Sarah Jean Pour Jan-Steffen Krüssel Udo Rudolf Markert Tanja Natascha Fehm Alexandra Petra Bielfeld Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells Mediators of Inflammation |
author_facet |
Dunja Maria Baston-Buest Olga Altergot-Ahmad Sarah Jean Pour Jan-Steffen Krüssel Udo Rudolf Markert Tanja Natascha Fehm Alexandra Petra Bielfeld |
author_sort |
Dunja Maria Baston-Buest |
title |
Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells |
title_short |
Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells |
title_full |
Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells |
title_fullStr |
Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells |
title_full_unstemmed |
Syndecan-1 Acts as an Important Regulator of CXCL1 Expression and Cellular Interaction of Human Endometrial Stromal and Trophoblast Cells |
title_sort |
syndecan-1 acts as an important regulator of cxcl1 expression and cellular interaction of human endometrial stromal and trophoblast cells |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2017-01-01 |
description |
Successful implantation of the embryo into the human receptive endometrium is substantial for the establishment of a healthy pregnancy. This study focusses on the role of Syndecan-1 at the embryo-maternal interface, the multitasking coreceptor influencing ligand concentration, release and receptor presentation, and cellular morphology. CXC motif ligand 1, being involved in chemotaxis and angiogenesis during implantation, is of special interest as a ligand of Syndecan-1. Human endometrial stromal cells with and without Syndecan-1 knock-down were decidualized and treated with specific inhibitors to evaluate signaling pathways regulating CXC ligand 1 expression. Western blot analyses of MAPK and Wnt members were performed, followed by analysis of spheroid interactions between human endometrial cells and extravillous trophoblast cells. By mimicking embryo contact using IL-1β, we showed less ERK and c-Jun activation by depletion of Syndecan-1 and less Frizzled 4 production as part of the canonical Wnt pathway. Additionally, more beta-catenin was phosphorylated and therefore degraded after depletion of Syndecan-1. Secretion of CXC motif ligand 1 depends on MEK-1 with respect to Syndecan-1. Regarding the interaction of endometrial and trophoblast cells, the spheroid center-to-center distances were smaller after depletion of Syndecan-1. Therefore, Syndecan-1 seems to affect signaling processes relevant to signaling and intercellular interaction at the trophoblast-decidual interface. |
url |
http://dx.doi.org/10.1155/2017/8379256 |
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