Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1
Duck hepatitis A virus type 1 (DHAV-1) is one of the most common and lethal pathogens in young ducklings. Live-attenuated DHAV vaccine (CH60 strain) developed by passaging in chicken embryos provided effective immune protection for ducklings. However, the accurate mechanism for such adaption in chic...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.01845/full |
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doaj-52317b2ff0bb4e718822f118b635a75a |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Jinyan Xie Jinyan Xie Qiurui Zeng Mingshu Wang Mingshu Wang Mingshu Wang Xumin Ou Xumin Ou Yunchao Ma Yunchao Ma Anchun Cheng Anchun Cheng Anchun Cheng Xin-Xin Zhao Xin-Xin Zhao Xin-Xin Zhao Mafeng Liu Mafeng Liu Mafeng Liu Dekang Zhu Dekang Zhu Shun Chen Shun Chen Shun Chen Renyong Jia Renyong Jia Renyong Jia Qiao Yang Qiao Yang Qiao Yang Ying Wu Ying Wu Ying Wu Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Yunya Liu Yunya Liu Yunya Liu Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Xiaoyue Chen Xiaoyue Chen Xiaoyue Chen |
| spellingShingle |
Jinyan Xie Jinyan Xie Qiurui Zeng Mingshu Wang Mingshu Wang Mingshu Wang Xumin Ou Xumin Ou Yunchao Ma Yunchao Ma Anchun Cheng Anchun Cheng Anchun Cheng Xin-Xin Zhao Xin-Xin Zhao Xin-Xin Zhao Mafeng Liu Mafeng Liu Mafeng Liu Dekang Zhu Dekang Zhu Shun Chen Shun Chen Shun Chen Renyong Jia Renyong Jia Renyong Jia Qiao Yang Qiao Yang Qiao Yang Ying Wu Ying Wu Ying Wu Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Yunya Liu Yunya Liu Yunya Liu Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Xiaoyue Chen Xiaoyue Chen Xiaoyue Chen Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1 Frontiers in Immunology Duck hepatitis A virus type 1 transcriptomic analysis chicken embryo models innate immune system SOCS |
| author_facet |
Jinyan Xie Jinyan Xie Qiurui Zeng Mingshu Wang Mingshu Wang Mingshu Wang Xumin Ou Xumin Ou Yunchao Ma Yunchao Ma Anchun Cheng Anchun Cheng Anchun Cheng Xin-Xin Zhao Xin-Xin Zhao Xin-Xin Zhao Mafeng Liu Mafeng Liu Mafeng Liu Dekang Zhu Dekang Zhu Shun Chen Shun Chen Shun Chen Renyong Jia Renyong Jia Renyong Jia Qiao Yang Qiao Yang Qiao Yang Ying Wu Ying Wu Ying Wu Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Yunya Liu Yunya Liu Yunya Liu Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Xiaoyue Chen Xiaoyue Chen Xiaoyue Chen |
| author_sort |
Jinyan Xie |
| title |
Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1 |
| title_short |
Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1 |
| title_full |
Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1 |
| title_fullStr |
Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1 |
| title_full_unstemmed |
Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1 |
| title_sort |
transcriptomic characterization of a chicken embryo model infected with duck hepatitis a virus type 1 |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Immunology |
| issn |
1664-3224 |
| publishDate |
2018-08-01 |
| description |
Duck hepatitis A virus type 1 (DHAV-1) is one of the most common and lethal pathogens in young ducklings. Live-attenuated DHAV vaccine (CH60 strain) developed by passaging in chicken embryos provided effective immune protection for ducklings. However, the accurate mechanism for such adaption in chicken embryos is not fully revealed. Here, we utilize RNA-sequencing to perform global transcriptional analysis of DHAV-1-innoculated embryonated livers along with histopathological and ultrastructural analysis. This study revealed that infection with DHAV-1 strain CH60 is associated with enhanced type I and II interferon responses, activated innate immune responses, elevated levels of suppressor of cytokine signaling 1 and 3 (SOCS1 and SOCS3) accompanied with abnormalities in multiple metabolic pathways. Excessive inflammatory and innate immune responses induced by the CH60 strain are related to severe liver damage. Our study presents a comprehensive characterization of the transcriptome of chicken embryos infected with DHAV-CH60 and provides insight for in-depth exploration of viral adaption and virus–host interactions. |
| topic |
Duck hepatitis A virus type 1 transcriptomic analysis chicken embryo models innate immune system SOCS |
| url |
https://www.frontiersin.org/article/10.3389/fimmu.2018.01845/full |
| work_keys_str_mv |
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doaj-52317b2ff0bb4e718822f118b635a75a2020-11-24T21:35:14ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-08-01910.3389/fimmu.2018.01845375804Transcriptomic Characterization of a Chicken Embryo Model Infected With Duck Hepatitis A Virus Type 1Jinyan Xie0Jinyan Xie1Qiurui Zeng2Mingshu Wang3Mingshu Wang4Mingshu Wang5Xumin Ou6Xumin Ou7Yunchao Ma8Yunchao Ma9Anchun Cheng10Anchun Cheng11Anchun Cheng12Xin-Xin Zhao13Xin-Xin Zhao14Xin-Xin Zhao15Mafeng Liu16Mafeng Liu17Mafeng Liu18Dekang Zhu19Dekang Zhu20Shun Chen21Shun Chen22Shun Chen23Renyong Jia24Renyong Jia25Renyong Jia26Qiao Yang27Qiao Yang28Qiao Yang29Ying Wu30Ying Wu31Ying Wu32Shaqiu Zhang33Shaqiu Zhang34Shaqiu Zhang35Yunya Liu36Yunya Liu37Yunya Liu38Yanling Yu39Yanling Yu40Yanling Yu41Ling Zhang42Ling Zhang43Ling Zhang44Xiaoyue Chen45Xiaoyue Chen46Xiaoyue Chen47Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaSchool of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, ChinaDuck hepatitis A virus type 1 (DHAV-1) is one of the most common and lethal pathogens in young ducklings. Live-attenuated DHAV vaccine (CH60 strain) developed by passaging in chicken embryos provided effective immune protection for ducklings. However, the accurate mechanism for such adaption in chicken embryos is not fully revealed. Here, we utilize RNA-sequencing to perform global transcriptional analysis of DHAV-1-innoculated embryonated livers along with histopathological and ultrastructural analysis. This study revealed that infection with DHAV-1 strain CH60 is associated with enhanced type I and II interferon responses, activated innate immune responses, elevated levels of suppressor of cytokine signaling 1 and 3 (SOCS1 and SOCS3) accompanied with abnormalities in multiple metabolic pathways. Excessive inflammatory and innate immune responses induced by the CH60 strain are related to severe liver damage. Our study presents a comprehensive characterization of the transcriptome of chicken embryos infected with DHAV-CH60 and provides insight for in-depth exploration of viral adaption and virus–host interactions.https://www.frontiersin.org/article/10.3389/fimmu.2018.01845/fullDuck hepatitis A virus type 1transcriptomic analysischicken embryo modelsinnate immune systemSOCS |