Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, India
Introduction: Globally, the Coronavirus Disease-2019 (COVID19) pandemic poses a high risk for Healthcare Workers (HCWs) who are among the population that is most vulnerable of being infected with Severe Acute Respiratory SyndromeCoronavirus-2 (SARS-CoV-2). With a prevailing pandemic such as COVID...
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doaj-523d5128f3b249ba96a0393f9026257d2021-06-18T08:27:14ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2021-06-01156DC22DC2610.7860/JCDR/2021/48889.14995Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, IndiaTherese MARY Dhason0Suganthi MANIKESI1Usha Krishnan2Vasant hamani3Hemalatha4C Devi5Nishat6B Manjula7Associate Professor, Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Associate Professor, Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Associate Professor, Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Dean, Department of Obstetrics and Gynaecology , Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Assistant Professor, Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Assistant Professor, Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Tutor, Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Medical Laboratory Technician, Department of Microbiology, Government Kilpauk Medical College, Chennai, Tamil Nadu, India.Introduction: Globally, the Coronavirus Disease-2019 (COVID19) pandemic poses a high risk for Healthcare Workers (HCWs) who are among the population that is most vulnerable of being infected with Severe Acute Respiratory SyndromeCoronavirus-2 (SARS-CoV-2). With a prevailing pandemic such as COVID-19, it becomes important to understand the presence and persistence of antibodies in the serum of HCW, testing positive for COVID-19 on Reverse transcriptase Polymerase Chain Reaction (RT-PCR). An understanding of the prevalence of IgG antibodies against COVID-19 and the duration for which they are present in the serum will help in predicting the immune response of individuals against the disease. Aim: To study the prevalence of COVID-19 IgG antibodies in laboratory confirmed COVID-19 RT-PCR positive symptomatic, asymptomatic and RT-PCR negative subjects. Materials and Methods: The present longitudinal study was conducted from April 2020 to December 2020 with a sample size of 90 participants based on a pilot study. Blood sample was collected and serum was separated. Enzyme Linked Immunosorbent Assay (ELISA) was done to detect the presence of COVID-19 IgG antibody in serum. Chi-square test and Pearson correlation were used to find out the statistical significance of COVID-19 IgG antibodies in COVID-19 positive and negative HCWs and the relationship between Cycle threshold values (Ct) and antibody levels, respectively. Results: Fever with sore throat was the most common (33%) symptom. Chi-square test done to compare IgG among RT-PCR positive and negative subjects showed p-value of <0.00001 which was significant. However, statistical significance was not found (p-value 0.9973) with respect to COVID-19 IgG antibodies in RT-PCR positive COVID-19 asymptomatic and symptomatic subjects. Mean antibody index in symptomatic and asymptomatic was 3.7743±1.9834 and 3.571±1.7961, respectively. Average number of days, the antibodies persisted was 25 days-266 days. Conclusion: The prevalence of COVID-19 antibodies among RT-PCR positive symptomatic, asymptomatic and RT-PCR negative subjects was 88%, 67% and 27%, respectively. The maximum number of days antibodies persisted was 266 days. Further studies will elucidate whether these antibodies prevent re-infection.https://www.jcdr.net/articles/PDF/14995/48889_CE[Ra1]_F[SK]_PF1(AB_SL)_PN(KM).pdfbreathlessnesscovid-19immune responseimmunoglobulinspolymerase chain reactiontransmission |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Therese MARY Dhason Suganthi MANIKESI Usha Krishnan Vasant hamani Hemalatha C Devi Nishat B Manjula |
spellingShingle |
Therese MARY Dhason Suganthi MANIKESI Usha Krishnan Vasant hamani Hemalatha C Devi Nishat B Manjula Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, India Journal of Clinical and Diagnostic Research breathlessness covid-19 immune response immunoglobulins polymerase chain reaction transmission |
author_facet |
Therese MARY Dhason Suganthi MANIKESI Usha Krishnan Vasant hamani Hemalatha C Devi Nishat B Manjula |
author_sort |
Therese MARY Dhason |
title |
Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, India |
title_short |
Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, India |
title_full |
Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, India |
title_fullStr |
Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, India |
title_full_unstemmed |
Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, India |
title_sort |
evaluation of igg antibody response to severe acute respiratory syndrome coronavirus-2 in healthcare workers in a tertiary care centre, chennai, india |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2021-06-01 |
description |
Introduction: Globally, the Coronavirus Disease-2019 (COVID19) pandemic poses a high risk for Healthcare Workers (HCWs)
who are among the population that is most vulnerable of
being infected with Severe Acute Respiratory SyndromeCoronavirus-2 (SARS-CoV-2). With a prevailing pandemic such
as COVID-19, it becomes important to understand the presence
and persistence of antibodies in the serum of HCW, testing
positive for COVID-19 on Reverse transcriptase Polymerase
Chain Reaction (RT-PCR). An understanding of the prevalence
of IgG antibodies against COVID-19 and the duration for which
they are present in the serum will help in predicting the immune
response of individuals against the disease.
Aim: To study the prevalence of COVID-19 IgG antibodies in
laboratory confirmed COVID-19 RT-PCR positive symptomatic,
asymptomatic and RT-PCR negative subjects.
Materials and Methods: The present longitudinal study was
conducted from April 2020 to December 2020 with a sample
size of 90 participants based on a pilot study. Blood sample
was collected and serum was separated. Enzyme Linked
Immunosorbent Assay (ELISA) was done to detect the presence
of COVID-19 IgG antibody in serum. Chi-square test and Pearson
correlation were used to find out the statistical significance of
COVID-19 IgG antibodies in COVID-19 positive and negative
HCWs and the relationship between Cycle threshold values (Ct)
and antibody levels, respectively.
Results: Fever with sore throat was the most common (33%)
symptom. Chi-square test done to compare IgG among RT-PCR
positive and negative subjects showed p-value of <0.00001
which was significant. However, statistical significance was
not found (p-value 0.9973) with respect to COVID-19 IgG
antibodies in RT-PCR positive COVID-19 asymptomatic and
symptomatic subjects. Mean antibody index in symptomatic
and asymptomatic was 3.7743±1.9834 and 3.571±1.7961,
respectively. Average number of days, the antibodies persisted
was 25 days-266 days.
Conclusion: The prevalence of COVID-19 antibodies among
RT-PCR positive symptomatic, asymptomatic and RT-PCR
negative subjects was 88%, 67% and 27%, respectively. The
maximum number of days antibodies persisted was 266 days.
Further studies will elucidate whether these antibodies prevent
re-infection. |
topic |
breathlessness covid-19 immune response immunoglobulins polymerase chain reaction transmission |
url |
https://www.jcdr.net/articles/PDF/14995/48889_CE[Ra1]_F[SK]_PF1(AB_SL)_PN(KM).pdf |
work_keys_str_mv |
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