The Exogenous Estrogen Antiproliferaive Effects On Cervical Cancer Cell Line: An In Vitro Study

• Main Authors: Zohreh Haghri, Rahim Ahmadi
• Format: Article
• Language: English
• Published: Islamic Azad University 2016-12-01
• Series: Journal of Pharmaceutical and Health Sciences
• Subjects: Estradiol; Hela cell line; Viability; Antitumor; cervical cancer
• Online Access: http://www.jphs.ir/article_527819.html
• ISSN: 2228-6780; 2228-6780

Estradiol valerate (Exogenous estrogen) is a synthetic ester and is also a female estrogen hormone. There are studies showing that estradiol may have anticancer effects. This study was performed to reveal the effects of exogenous estrogens on the viability of cervical cancer cells in cell culture. We used Hela cells as our cell line in this study. Hela cells were exposed to 0.0001, 0.001, 0.01, 0.1, 1 and 10 mg/ml of estradiol. Control Hela cells were not exposed to estradiol. MTT assay was used to determine the viability of cervical cancer cells in cell culture. Results indicated that administration of 10 mg/ml Estradiol led to significant decrease in viability of Hela cells compared to control cells (P<0.05). Administration of 0.0001, 0.001, 0.01, 0.1 and 1 mg/ml of estradiol did not change the viability of Hela cells significantly compared to control group. The results are expressed as the mean ± SD of more than 3 independently performed experiments. Statistical significance was set at p<0.05. Motivated by the previous finding that consumption of steroid derivates correlated with cancer cell growth rate, According to the findings, high doses of estradiol have cytotoxic effects on cervical cancer cells. There are no studies revealing that estradiol may reduce the viability of cervical cancer cells.