Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage.
We had previously reported that exchange protein for cAMP 1 (Epac1) reduced inflammatory mediators in the retina of mice and in retinal endothelial cells (REC). Since ischemia can induce retinal damage potentially through activation of inflammatory cascades, we hypothesized that Epac1 would protect...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2018-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC6147720?pdf=render |
id |
doaj-52623ee8805d41ff841f37df3f122905 |
---|---|
record_format |
Article |
spelling |
doaj-52623ee8805d41ff841f37df3f1229052020-11-25T02:11:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01139e020434610.1371/journal.pone.0204346Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage.Li LiuYoude JiangJena J SteinleWe had previously reported that exchange protein for cAMP 1 (Epac1) reduced inflammatory mediators in the retina of mice and in retinal endothelial cells (REC). Since ischemia can induce retinal damage potentially through activation of inflammatory cascades, we hypothesized that Epac1 would protect the retina against neuronal and vascular damage after exposure to ischemia/reperfusion (I/R). We used Epac1 floxed and endothelial cell specific Epac1 knockout mice for this work. We exposed them to ischemia for 90 minutes followed by reperfusion. One day after I/R, some mice were used for fluorescein angiography imaging or Evan's blue measurements of permeability. Mice were sacrificed at 2 days for neuronal measurements and at 10 days for measurements of degenerate capillaries. Data show increased leakage in the Epac1 Cre-Lox (Epac1 EC-KO) mice exposed to I/R when compared to Epac1 floxed mice with the same treatment. I/R also increased numbers of degenerate capillaries and cell loss in all retinal layers of Epac1 EC-KO mice. Retinal thickness was reduced more significantly in the Epac1 EC-KO mice compared to Epac1 floxed mice after I/R. Taken together, the data suggest that Epac1 is protective against both neuronal and vascular damage to the retina after exposure to I/R.http://europepmc.org/articles/PMC6147720?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Liu Youde Jiang Jena J Steinle |
spellingShingle |
Li Liu Youde Jiang Jena J Steinle Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage. PLoS ONE |
author_facet |
Li Liu Youde Jiang Jena J Steinle |
author_sort |
Li Liu |
title |
Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage. |
title_short |
Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage. |
title_full |
Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage. |
title_fullStr |
Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage. |
title_full_unstemmed |
Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage. |
title_sort |
epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
We had previously reported that exchange protein for cAMP 1 (Epac1) reduced inflammatory mediators in the retina of mice and in retinal endothelial cells (REC). Since ischemia can induce retinal damage potentially through activation of inflammatory cascades, we hypothesized that Epac1 would protect the retina against neuronal and vascular damage after exposure to ischemia/reperfusion (I/R). We used Epac1 floxed and endothelial cell specific Epac1 knockout mice for this work. We exposed them to ischemia for 90 minutes followed by reperfusion. One day after I/R, some mice were used for fluorescein angiography imaging or Evan's blue measurements of permeability. Mice were sacrificed at 2 days for neuronal measurements and at 10 days for measurements of degenerate capillaries. Data show increased leakage in the Epac1 Cre-Lox (Epac1 EC-KO) mice exposed to I/R when compared to Epac1 floxed mice with the same treatment. I/R also increased numbers of degenerate capillaries and cell loss in all retinal layers of Epac1 EC-KO mice. Retinal thickness was reduced more significantly in the Epac1 EC-KO mice compared to Epac1 floxed mice after I/R. Taken together, the data suggest that Epac1 is protective against both neuronal and vascular damage to the retina after exposure to I/R. |
url |
http://europepmc.org/articles/PMC6147720?pdf=render |
work_keys_str_mv |
AT liliu epac1protectstheretinaagainstischemiareperfusioninducedneuronalandvasculardamage AT youdejiang epac1protectstheretinaagainstischemiareperfusioninducedneuronalandvasculardamage AT jenajsteinle epac1protectstheretinaagainstischemiareperfusioninducedneuronalandvasculardamage |
_version_ |
1724915364316315648 |