Degradation or excretion of quantum dots in mouse embryonic stem cells
<p>Abstract</p> <p>Background</p> <p>Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study...
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doaj-52673d5a943e4a86b606244532139aa42020-11-25T03:13:34ZengBMCBMC Biotechnology1472-67502010-05-011013610.1186/1472-6750-10-36Degradation or excretion of quantum dots in mouse embryonic stem cellsLiu WeiZhou GuangZhang WenPi QingCao Yilin<p>Abstract</p> <p>Background</p> <p>Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells.</p> <p>Results</p> <p>Mouse embryonic stem cells (ESCs) and mouse embryonic fibroblasts (MEFs) were labeled with QD 655. QD-labeling was monitored by fluorescence microscopy and flow cytometry for 72 hours. Both types of cells were labeled efficiently, but a quick loss of QD-labeling in ESCs was observed within 48 hours, which was not prevented by inhibiting cell proliferation. Transmission electron microscope analysis showed a dramatic decrease of QD number in vesicles of ESCs at 24 hours post-labeling, suggesting that QDs might be degraded. In addition, supernatants collected from labeled ESCs in culture were used to label cells again, indicating that some QDs were excreted from cells.</p> <p>Conclusion</p> <p>This is the first study to demonstrate that the metabolism of QDs in different type of cells is different. QDs were quickly degraded or excreted from ESCs after labeling.</p> http://www.biomedcentral.com/1472-6750/10/36 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liu Wei Zhou Guang Zhang Wen Pi Qing Cao Yilin |
spellingShingle |
Liu Wei Zhou Guang Zhang Wen Pi Qing Cao Yilin Degradation or excretion of quantum dots in mouse embryonic stem cells BMC Biotechnology |
author_facet |
Liu Wei Zhou Guang Zhang Wen Pi Qing Cao Yilin |
author_sort |
Liu Wei |
title |
Degradation or excretion of quantum dots in mouse embryonic stem cells |
title_short |
Degradation or excretion of quantum dots in mouse embryonic stem cells |
title_full |
Degradation or excretion of quantum dots in mouse embryonic stem cells |
title_fullStr |
Degradation or excretion of quantum dots in mouse embryonic stem cells |
title_full_unstemmed |
Degradation or excretion of quantum dots in mouse embryonic stem cells |
title_sort |
degradation or excretion of quantum dots in mouse embryonic stem cells |
publisher |
BMC |
series |
BMC Biotechnology |
issn |
1472-6750 |
publishDate |
2010-05-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells.</p> <p>Results</p> <p>Mouse embryonic stem cells (ESCs) and mouse embryonic fibroblasts (MEFs) were labeled with QD 655. QD-labeling was monitored by fluorescence microscopy and flow cytometry for 72 hours. Both types of cells were labeled efficiently, but a quick loss of QD-labeling in ESCs was observed within 48 hours, which was not prevented by inhibiting cell proliferation. Transmission electron microscope analysis showed a dramatic decrease of QD number in vesicles of ESCs at 24 hours post-labeling, suggesting that QDs might be degraded. In addition, supernatants collected from labeled ESCs in culture were used to label cells again, indicating that some QDs were excreted from cells.</p> <p>Conclusion</p> <p>This is the first study to demonstrate that the metabolism of QDs in different type of cells is different. QDs were quickly degraded or excreted from ESCs after labeling.</p> |
url |
http://www.biomedcentral.com/1472-6750/10/36 |
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