Reduction and esterification of cholesterol and sitosterol by homogenates of feces

Mixtures of cholesterol-1,2-3H and sitosterol-4-14C have been incubated with suspensions of feces in order to compare the behavior of the phytosterol with transformations known to take place with cholesterol under these conditions. Within the limitations of the study, both labeled sterols were ester...

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Main Authors:	R.S. ROSENFELD, LEON HELLMAN
Format:	Article
Language:	English
Published:	Elsevier 1971-03-01
Series:	Journal of Lipid Research
Subjects:	feces sterols
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520395298

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spelling	doaj-526cb32d5c924df1a53fce72708b00442021-04-24T05:52:11ZengElsevierJournal of Lipid Research0022-22751971-03-01122192197Reduction and esterification of cholesterol and sitosterol by homogenates of fecesR.S. ROSENFELD0LEON HELLMAN1Institute for Steroid Research and Department of Oncology, Montefiore Hospital and Medical Center, New York 10467Institute for Steroid Research and Department of Oncology, Montefiore Hospital and Medical Center, New York 10467Mixtures of cholesterol-1,2-3H and sitosterol-4-14C have been incubated with suspensions of feces in order to compare the behavior of the phytosterol with transformations known to take place with cholesterol under these conditions. Within the limitations of the study, both labeled sterols were esterified to the same extent, and reduction of the Δ5 double bond to the saturated analogue proceeded equally in both substances. After correcting for procedural losses, the recoveries of 3H and 14C from the incubations were always less than the controls; this strongly indicates destruction of sterol by feces microorganisms.http://www.sciencedirect.com/science/article/pii/S0022227520395298feces sterols
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	R.S. ROSENFELD LEON HELLMAN
spellingShingle	R.S. ROSENFELD LEON HELLMAN Reduction and esterification of cholesterol and sitosterol by homogenates of feces Journal of Lipid Research feces sterols
author_facet	R.S. ROSENFELD LEON HELLMAN
author_sort	R.S. ROSENFELD
title	Reduction and esterification of cholesterol and sitosterol by homogenates of feces
title_short	Reduction and esterification of cholesterol and sitosterol by homogenates of feces
title_full	Reduction and esterification of cholesterol and sitosterol by homogenates of feces
title_fullStr	Reduction and esterification of cholesterol and sitosterol by homogenates of feces
title_full_unstemmed	Reduction and esterification of cholesterol and sitosterol by homogenates of feces
title_sort	reduction and esterification of cholesterol and sitosterol by homogenates of feces
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1971-03-01
description	Mixtures of cholesterol-1,2-3H and sitosterol-4-14C have been incubated with suspensions of feces in order to compare the behavior of the phytosterol with transformations known to take place with cholesterol under these conditions. Within the limitations of the study, both labeled sterols were esterified to the same extent, and reduction of the Δ5 double bond to the saturated analogue proceeded equally in both substances. After correcting for procedural losses, the recoveries of 3H and 14C from the incubations were always less than the controls; this strongly indicates destruction of sterol by feces microorganisms.
topic	feces sterols
url	http://www.sciencedirect.com/science/article/pii/S0022227520395298
work_keys_str_mv	AT rsrosenfeld reductionandesterificationofcholesterolandsitosterolbyhomogenatesoffeces AT leonhellman reductionandesterificationofcholesterolandsitosterolbyhomogenatesoffeces
_version_	1721511565326286848

Reduction and esterification of cholesterol and sitosterol by homogenates of feces

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