Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice

Abstract Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects multiple organs. Recent studies suggest relevance between cysteine protease cathepsin S (CTSS) expression and SLE. To investigate the mechanism of CTSS in SLE, CTSS-overexpressing transgenic (TG) mice were gener...

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Main Authors:	Jinhee Lee, Soyoung Jang, Minjee Choi, Mincheol Kang, Su-Geun Lim, SI-Yong Kim, Soyeon Jang, Jiwon Ko, Eungyung Kim, Junkoo Yi, Yeonsik Choo, Myoung Ok Kim, Zae Young Ryoo
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-08-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-021-94855-5

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spelling	doaj-527c6365fe894cf79ff097e3b7400ebd2021-08-15T11:27:59ZengNature Publishing GroupScientific Reports2045-23222021-08-011111810.1038/s41598-021-94855-5Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic miceJinhee Lee0Soyoung Jang1Minjee Choi2Mincheol Kang3Su-Geun Lim4SI-Yong Kim5Soyeon Jang6Jiwon Ko7Eungyung Kim8Junkoo Yi9Yeonsik Choo10Myoung Ok Kim11Zae Young Ryoo12School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversityCore Protein Resources Center, DGISTDepartment of Physiology and Cell Biology, School of Medicine, University of Nevada RenoSchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversityDepartment of Animal Science and Biotechnology, Kyungpook National UniversityGyeongsangbukdo Livestock Research InstituteDepartment of Biology, Kyungpook National UniversityDepartment of Animal Science and Biotechnology, Kyungpook National UniversitySchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversityAbstract Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects multiple organs. Recent studies suggest relevance between cysteine protease cathepsin S (CTSS) expression and SLE. To investigate the mechanism of CTSS in SLE, CTSS-overexpressing transgenic (TG) mice were generated, and induced lupus-like symptoms. Eight months later, the TG mice spontaneously developed typical SLE symptoms regardless of the inducement. Furthermore, we observed increased toll-like receptor 7 (TLR7) expression with increased monocyte and neutrophil populations in the TG mice. In conclusion, overexpression of CTSS in mice influences TLR7 expression, autoantibodies and IFN-α, which leads to an autoimmune reaction and exacerbates lupus-like symptoms.https://doi.org/10.1038/s41598-021-94855-5
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jinhee Lee Soyoung Jang Minjee Choi Mincheol Kang Su-Geun Lim SI-Yong Kim Soyeon Jang Jiwon Ko Eungyung Kim Junkoo Yi Yeonsik Choo Myoung Ok Kim Zae Young Ryoo
spellingShingle	Jinhee Lee Soyoung Jang Minjee Choi Mincheol Kang Su-Geun Lim SI-Yong Kim Soyeon Jang Jiwon Ko Eungyung Kim Junkoo Yi Yeonsik Choo Myoung Ok Kim Zae Young Ryoo Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice Scientific Reports
author_facet	Jinhee Lee Soyoung Jang Minjee Choi Mincheol Kang Su-Geun Lim SI-Yong Kim Soyeon Jang Jiwon Ko Eungyung Kim Junkoo Yi Yeonsik Choo Myoung Ok Kim Zae Young Ryoo
author_sort	Jinhee Lee
title	Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice
title_short	Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice
title_full	Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice
title_fullStr	Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice
title_full_unstemmed	Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice
title_sort	overexpression of cathepsin s exacerbates lupus pathogenesis through upregulation tlr7 and ifn-α in transgenic mice
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2021-08-01
description	Abstract Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects multiple organs. Recent studies suggest relevance between cysteine protease cathepsin S (CTSS) expression and SLE. To investigate the mechanism of CTSS in SLE, CTSS-overexpressing transgenic (TG) mice were generated, and induced lupus-like symptoms. Eight months later, the TG mice spontaneously developed typical SLE symptoms regardless of the inducement. Furthermore, we observed increased toll-like receptor 7 (TLR7) expression with increased monocyte and neutrophil populations in the TG mice. In conclusion, overexpression of CTSS in mice influences TLR7 expression, autoantibodies and IFN-α, which leads to an autoimmune reaction and exacerbates lupus-like symptoms.
url	https://doi.org/10.1038/s41598-021-94855-5
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Overexpression of cathepsin S exacerbates lupus pathogenesis through upregulation TLR7 and IFN-α in transgenic mice

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