A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>

A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>

A novel pathogenesis-related protein 4 (PR4) encoding gene, LcPR4a, was induced in Lens culinaris following Ascochyta lentis infection. LcPR4a encodes a predicted 146 amino acid protein of 15.8 kDa. The putative LcPR4a protein belongs to the class II PR4 family and has close phylogenetic affinity to...

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Main Authors: Niloofar VAGHEFI, Barkat MUSTAFA, Nabeen DULAL, Jamie SELBY-PHAM, Paul TAYLOR, Rebecca FORD
Format: Article
Language:English
Published: Firenze University Press 2013-04-01
Series:Phytopathologia Mediterranea
Subjects:
Ascochyta blight
PR4
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/5688
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spelling doaj-52a4c2ae53874460a6eeb828e235a1642020-11-25T03:46:28ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952013-04-0152110.14601/Phytopathol_Mediterr-1179111080A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>Niloofar VAGHEFI0Barkat MUSTAFANabeen DULALJamie SELBY-PHAMPaul TAYLORRebecca FORDthe University of MelbourneA novel pathogenesis-related protein 4 (PR4) encoding gene, LcPR4a, was induced in Lens culinaris following Ascochyta lentis infection. LcPR4a encodes a predicted 146 amino acid protein of 15.8 kDa. The putative LcPR4a protein belongs to the class II PR4 family and has close phylogenetic affinity to PR4 proteins from related species. qPCR analysis revealed differential expression of the LcPR4a gene upon Ascochyta lentis infection in both resistant and susceptible cultivars. This, combined with preliminary in vitro antifungal assays of the recombinant protein expressed in E. coli, suggests the potential important role of LcPR4a in the defence response of lentil to Ascochyta lentis attack.https://oajournals.fupress.net/index.php/pm/article/view/5688Ascochyta blightPR4
collection DOAJ
language English
format Article
sources DOAJ
author Niloofar VAGHEFI
Barkat MUSTAFA
Nabeen DULAL
Jamie SELBY-PHAM
Paul TAYLOR
Rebecca FORD
spellingShingle Niloofar VAGHEFI
Barkat MUSTAFA
Nabeen DULAL
Jamie SELBY-PHAM
Paul TAYLOR
Rebecca FORD
A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>
Phytopathologia Mediterranea
Ascochyta blight
PR4
author_facet Niloofar VAGHEFI
Barkat MUSTAFA
Nabeen DULAL
Jamie SELBY-PHAM
Paul TAYLOR
Rebecca FORD
author_sort Niloofar VAGHEFI
title A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>
title_short A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>
title_full A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>
title_fullStr A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>
title_full_unstemmed A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against <em>Ascochyta lentis</em>
title_sort novel pathogenesis-related protein (lcpr4a) from lentil, and its involvement in defence against <em>ascochyta lentis</em>
publisher Firenze University Press
series Phytopathologia Mediterranea
issn 0031-9465
1593-2095
publishDate 2013-04-01
description A novel pathogenesis-related protein 4 (PR4) encoding gene, LcPR4a, was induced in Lens culinaris following Ascochyta lentis infection. LcPR4a encodes a predicted 146 amino acid protein of 15.8 kDa. The putative LcPR4a protein belongs to the class II PR4 family and has close phylogenetic affinity to PR4 proteins from related species. qPCR analysis revealed differential expression of the LcPR4a gene upon Ascochyta lentis infection in both resistant and susceptible cultivars. This, combined with preliminary in vitro antifungal assays of the recombinant protein expressed in E. coli, suggests the potential important role of LcPR4a in the defence response of lentil to Ascochyta lentis attack.
topic Ascochyta blight
PR4
url https://oajournals.fupress.net/index.php/pm/article/view/5688
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