Preliminary study on the galectin molecular diversity in Moroccoan Phlebotomus papatasi sandfly populations

• Main Author: S. Guernaoui, D. Garcia, A. Boumezzough, D. Fontenille & D. Sereno
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2011-06-01
• Series: Journal of Vector Borne Diseases
• Subjects: Galectin binding protein; Leishmania major; molecular diversity; Phlebotomus paptas
• Online Access: http://www.mrcindia.org/journal/issues/482067.pdf

Context: Galactose binding protein (PpGalec) plays an important role in the specificity of Phlebotomus papatasisandfly for Leishmania major. The molecular diversity of this ligand is currently unknown but might havesome influence on the ability of PpGalec to efficiently recognize L. major in natural sandfly populations.Objective: To explore the molecular diversity of the P. papatasi Galectin gene (PpGalec) in natural sandflypopulation of Morocco.Results & Conclusions: Sequence variations of PpGalec was analyzed in 31 P. papatasi specimens collectedfrom endemic and non-endemic zoonotic cutaneous leishmaniasis foci of Morocco. Among the 211 amino acidpositions analyzed, 11 are subjected to mutation. Interestingly, we observe that one mutation directly affect anamino acid known to be involved in the substrate recognition by galectin. The repercussion of this polymorphismon the capacity of the galectin to efficiently bind the L. major Lipophosphoglycane (LPG) awaits furtherinvestigations.