LINC02738 Participates in the Development of Kidney Cancer Through the miR-20b/Sox4 Axis

• Main Authors: Han C, Xu B, Zhou L, Li L, Lu C, Yu GP, Liu YS
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-10-01
• Series: OncoTargets and Therapy
• Subjects: linc02783; mir-20b; sox-4; renal cell carcinoma; the cancer genome atlas; prognosis
• Online Access: https://www.dovepress.com/linc02738-participates-in-the-development-of-kidney-cancer-through-the-peer-reviewed-article-OTT

Chao Han,* Bin Xu,* Lin Zhou, Long Li, Chao Lu, Guo-Peng Yu, Yu-Shan Liu Department of Urology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yu-Shan LiuDepartment of Urology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, No. 639 Zhizaoju Road, Shanghai 200011, People’s Republic of ChinaTel [phone]Email [e-mail]: Long non-coding RNAs (lncRNAs) can affect tumorigenesis. Data from The Cancer Genome Atlas (TCAG) suggest that LINC02783 is highly expressed in renal cell carcinoma (RCC) and is expected to be a potential biological target. We conducted this study to verify this.Patients and Methods: We conducted this study to verify the opinion that “LINC02783 is highly expressed in renal cell carcinoma (RCC) and is expected to be a potential biological target”. We employed quantitative real-time polymerase chain reaction (qRT-PCR) to test LINC02783 expression in RCC tissues, CKK-8 assay and transwell assay to assess the viability and invasion of RCC cells, Western blot to quantify Sox-4 expression, dual-luciferase reporter (DLR) assay and RNA immunoprecipitation (RIP) assay to analyze the interaction between LINC02783 and miR-20b, in vivo experiments to test tumor formation.Results: We detected high LINC02783 expression in RCC patients. Patients with higher LINC02783 levels had a markedly poorer prognosis. In vitro and in vivo, the down-regulation of LINC02783 suppressed the viability and invasion of RCC cells. The DLR assay results revealed that LINC02783 enhanced Sox-4 expression by regulating miR-20b. LINC02783 can act as a sponge for miR-20b to inhibit Sox-4 expression.Conclusion: LINC02783 is highly expressed in RCC patients and indicates a poor prognosis. LINC02783 can affect the occurrence and progression of RCC through the miR-20b/Sox-4 axis, making it a promising target for the treatment of RCC.Keywords: LINC02783, miR-20b, Sox-4, renal cell carcinoma, The Cancer Genome Atlas, prognosis