Antitumor effect of TW-37, a BH3 mimetic in human oral cancer
Abstract TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied yet. Here, we attempted to confirm antitumor activity of TW37 in human oral cancer...
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doaj-52b1cd287d124d66bf47f2630fe3ef0a2020-12-06T12:21:59ZengBMCLaboratory Animal Research2233-76602019-12-013511810.1186/s42826-019-0028-7Antitumor effect of TW-37, a BH3 mimetic in human oral cancerChi-Hyun Ahn0Won Woo Lee1Yun Chan Jung2Ji-Ae Shin3Kyoung-Ok Hong4Sujung Choi5Neeti Swarup6Jihoon Kim7Min-Hye Ahn8Minjung Jung9Sung-Dae Cho10Bohwan Jin11Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityLaboratory Animal Center, CHA University, CHA Biocomplex, Sampyeong-dongChaonDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityDepartment of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National UniversityLaboratory Animal Center, CHA University, CHA Biocomplex, Sampyeong-dongAbstract TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied yet. Here, we attempted to confirm antitumor activity of TW37 in human oral cancer. TW-37 significantly inhibited cell proliferation and increased the number of dead cells in MC-3 and HSC-3 human oral cancer cell lines. TW-37 enhanced apoptosis of both cell lines evidenced by annexin V/propidium iodide double staining, sub-G1 population analysis and the detection of cleaved poly (ADP-ribose) polymerase and caspase-3. In addition, TW-37 markedly downregulated the expression of Bcl-2 protein, while not affecting Bcl-xL or myeloid cell leukemia-1. In vivo, TW-37 inhibited tumor growth in a nude mice xenograft model without any significant liver and kidney toxicities. Collectively, these data reveal that TW-37 may be a promising small molecule to inhibit human oral cancer.https://doi.org/10.1186/s42826-019-0028-7TW-37ApoptosisBcl-2Oral cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chi-Hyun Ahn Won Woo Lee Yun Chan Jung Ji-Ae Shin Kyoung-Ok Hong Sujung Choi Neeti Swarup Jihoon Kim Min-Hye Ahn Minjung Jung Sung-Dae Cho Bohwan Jin |
spellingShingle |
Chi-Hyun Ahn Won Woo Lee Yun Chan Jung Ji-Ae Shin Kyoung-Ok Hong Sujung Choi Neeti Swarup Jihoon Kim Min-Hye Ahn Minjung Jung Sung-Dae Cho Bohwan Jin Antitumor effect of TW-37, a BH3 mimetic in human oral cancer Laboratory Animal Research TW-37 Apoptosis Bcl-2 Oral cancer |
author_facet |
Chi-Hyun Ahn Won Woo Lee Yun Chan Jung Ji-Ae Shin Kyoung-Ok Hong Sujung Choi Neeti Swarup Jihoon Kim Min-Hye Ahn Minjung Jung Sung-Dae Cho Bohwan Jin |
author_sort |
Chi-Hyun Ahn |
title |
Antitumor effect of TW-37, a BH3 mimetic in human oral cancer |
title_short |
Antitumor effect of TW-37, a BH3 mimetic in human oral cancer |
title_full |
Antitumor effect of TW-37, a BH3 mimetic in human oral cancer |
title_fullStr |
Antitumor effect of TW-37, a BH3 mimetic in human oral cancer |
title_full_unstemmed |
Antitumor effect of TW-37, a BH3 mimetic in human oral cancer |
title_sort |
antitumor effect of tw-37, a bh3 mimetic in human oral cancer |
publisher |
BMC |
series |
Laboratory Animal Research |
issn |
2233-7660 |
publishDate |
2019-12-01 |
description |
Abstract TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied yet. Here, we attempted to confirm antitumor activity of TW37 in human oral cancer. TW-37 significantly inhibited cell proliferation and increased the number of dead cells in MC-3 and HSC-3 human oral cancer cell lines. TW-37 enhanced apoptosis of both cell lines evidenced by annexin V/propidium iodide double staining, sub-G1 population analysis and the detection of cleaved poly (ADP-ribose) polymerase and caspase-3. In addition, TW-37 markedly downregulated the expression of Bcl-2 protein, while not affecting Bcl-xL or myeloid cell leukemia-1. In vivo, TW-37 inhibited tumor growth in a nude mice xenograft model without any significant liver and kidney toxicities. Collectively, these data reveal that TW-37 may be a promising small molecule to inhibit human oral cancer. |
topic |
TW-37 Apoptosis Bcl-2 Oral cancer |
url |
https://doi.org/10.1186/s42826-019-0028-7 |
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