Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea.
Rickettsiae are associated with a diverse range of invertebrate hosts. Of these, mosquitoes could emerge as one of the most important vectors because of their ability to transmit significant numbers of pathogens and parasites throughout the world. Recent studies have implicated Anopheles gambiae as...
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doaj-52c66c701c984e87b57e2f4e31901fb72020-11-25T01:53:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018832710.1371/journal.pone.0188327Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea.Alice N MainaTerry A KleinHeung-Chul KimSung-Tae ChongYu YangKristin MullinsJu JiangHeidi St JohnRichard G JarmanJun HangAllen L RichardsRickettsiae are associated with a diverse range of invertebrate hosts. Of these, mosquitoes could emerge as one of the most important vectors because of their ability to transmit significant numbers of pathogens and parasites throughout the world. Recent studies have implicated Anopheles gambiae as a potential vector of Rickettsia felis. Herein we report that a metagenome sequencing study identified rickettsial sequence reads in culicine mosquitoes from the Republic of Korea. The detected rickettsiae were characterized by a genus-specific quantitative real-time PCR assay and sequencing of rrs, gltA, 17kDa, ompB, and sca4 genes. Three novel rickettsial genotypes were detected (Rickettsia sp. A12.2646, Rickettsia sp. A12.2638 and Rickettsia sp. A12.3271), from Mansonia uniformis, Culex pipiens, and Aedes esoensis, respectively. The results underscore the need to determine the Rickettsia species diversity associated with mosquitoes, their evolution, distribution and pathogenic potential.http://europepmc.org/articles/PMC5695765?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alice N Maina Terry A Klein Heung-Chul Kim Sung-Tae Chong Yu Yang Kristin Mullins Ju Jiang Heidi St John Richard G Jarman Jun Hang Allen L Richards |
spellingShingle |
Alice N Maina Terry A Klein Heung-Chul Kim Sung-Tae Chong Yu Yang Kristin Mullins Ju Jiang Heidi St John Richard G Jarman Jun Hang Allen L Richards Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea. PLoS ONE |
author_facet |
Alice N Maina Terry A Klein Heung-Chul Kim Sung-Tae Chong Yu Yang Kristin Mullins Ju Jiang Heidi St John Richard G Jarman Jun Hang Allen L Richards |
author_sort |
Alice N Maina |
title |
Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea. |
title_short |
Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea. |
title_full |
Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea. |
title_fullStr |
Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea. |
title_full_unstemmed |
Molecular characterization of novel mosquito-borne Rickettsia spp. from mosquitoes collected at the Demilitarized Zone of the Republic of Korea. |
title_sort |
molecular characterization of novel mosquito-borne rickettsia spp. from mosquitoes collected at the demilitarized zone of the republic of korea. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Rickettsiae are associated with a diverse range of invertebrate hosts. Of these, mosquitoes could emerge as one of the most important vectors because of their ability to transmit significant numbers of pathogens and parasites throughout the world. Recent studies have implicated Anopheles gambiae as a potential vector of Rickettsia felis. Herein we report that a metagenome sequencing study identified rickettsial sequence reads in culicine mosquitoes from the Republic of Korea. The detected rickettsiae were characterized by a genus-specific quantitative real-time PCR assay and sequencing of rrs, gltA, 17kDa, ompB, and sca4 genes. Three novel rickettsial genotypes were detected (Rickettsia sp. A12.2646, Rickettsia sp. A12.2638 and Rickettsia sp. A12.3271), from Mansonia uniformis, Culex pipiens, and Aedes esoensis, respectively. The results underscore the need to determine the Rickettsia species diversity associated with mosquitoes, their evolution, distribution and pathogenic potential. |
url |
http://europepmc.org/articles/PMC5695765?pdf=render |
work_keys_str_mv |
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