<b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904
<p class="aresumo">The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of <em>dourado </em>samples from the middle Paranapanema river and...
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doaj-52d1c3ad6a184330bba339c3557232d32020-11-25T00:44:07ZengEditora da Universidade Estadual de Maringá (Eduem)Acta Scientiarum: Animal Sciences1806-26361807-86722013-03-0135211912610.4025/actascianimsci.v35i2.15904<b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904Rodolfo Nardez SirolMário Luís OrsiCarla Martins LopesFernanda Simões de AlmeidaLeda Maria Koelblinger Sodré<p class="aresumo">The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of <em>dourado </em>samples from the middle Paranapanema river and from the broodstock used in the stock enhancement program of the Aquaculture and Hydrobiology Station at the Salto Grande Hydroelectric Power Plant. Nineteen RAPD primers were analyzed, which generated 299 bands and the latter were used for genetic analysis. The percentage of polymorphic fragments was higher in stock captured in the Paranapanema river than from fry stocks. The genetic diversity was lower in the broodstock than in natural population. Amova results showed that most inter-population genetic variation lay within stocks (83.9%) and not between them (16.1%). Moderate genetic differentiation (<em>F</em><sub>ST</sub> = 0.16) was reported. Nevertheless, differentiation decreased when the four fry stocks were mixed and analyzed as a single population (<em>F</em><sub>ST</sub> = 0.07). Instead of releasing each lot separately into the water, mixing specimens produced in the various fry stocks before releasing them in the river would be more feasible. The restocked population will have a genetic structure closer to natural populations.</p> <p class="apalavrachave"> </p>http://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/15904douradogenetic variabilityhatchery stocksfry stocks |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rodolfo Nardez Sirol Mário Luís Orsi Carla Martins Lopes Fernanda Simões de Almeida Leda Maria Koelblinger Sodré |
spellingShingle |
Rodolfo Nardez Sirol Mário Luís Orsi Carla Martins Lopes Fernanda Simões de Almeida Leda Maria Koelblinger Sodré <b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904 Acta Scientiarum: Animal Sciences dourado genetic variability hatchery stocks fry stocks |
author_facet |
Rodolfo Nardez Sirol Mário Luís Orsi Carla Martins Lopes Fernanda Simões de Almeida Leda Maria Koelblinger Sodré |
author_sort |
Rodolfo Nardez Sirol |
title |
<b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904 |
title_short |
<b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904 |
title_full |
<b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904 |
title_fullStr |
<b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904 |
title_full_unstemmed |
<b>Genetic monitoring by rapd markers for repopulation programs of <i>Salminus brasiliensis</i> (Pisces, Characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904 |
title_sort |
<b>genetic monitoring by rapd markers for repopulation programs of <i>salminus brasiliensis</i> (pisces, characiformes)</b> - doi: 10.4025/actascianimsci.v35i2.15904 |
publisher |
Editora da Universidade Estadual de Maringá (Eduem) |
series |
Acta Scientiarum: Animal Sciences |
issn |
1806-2636 1807-8672 |
publishDate |
2013-03-01 |
description |
<p class="aresumo">The preservation of the genetic variability of hatchery stocks used to supplement natural populations is a priority. Current study employed RAPD markers to examine the genetic diversity of <em>dourado </em>samples from the middle Paranapanema river and from the broodstock used in the stock enhancement program of the Aquaculture and Hydrobiology Station at the Salto Grande Hydroelectric Power Plant. Nineteen RAPD primers were analyzed, which generated 299 bands and the latter were used for genetic analysis. The percentage of polymorphic fragments was higher in stock captured in the Paranapanema river than from fry stocks. The genetic diversity was lower in the broodstock than in natural population. Amova results showed that most inter-population genetic variation lay within stocks (83.9%) and not between them (16.1%). Moderate genetic differentiation (<em>F</em><sub>ST</sub> = 0.16) was reported. Nevertheless, differentiation decreased when the four fry stocks were mixed and analyzed as a single population (<em>F</em><sub>ST</sub> = 0.07). Instead of releasing each lot separately into the water, mixing specimens produced in the various fry stocks before releasing them in the river would be more feasible. The restocked population will have a genetic structure closer to natural populations.</p> <p class="apalavrachave"> </p> |
topic |
dourado genetic variability hatchery stocks fry stocks |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/15904 |
work_keys_str_mv |
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