Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosis
Abstract Background As a prevalent type of cryptogenic fibrotic disease with high mortality, idiopathic pulmonary fibrosis (IPF) still lacks effective therapeutic drugs. The compounds extracted from buds and flowers of Chrysanthemum indicum Linné with supercritical-carbon dioxide fluid (CISCFE) has...
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doaj-52fec923ec044cf99a72225def5a6d672021-09-26T11:49:38ZengBMCBMC Complementary Medicine and Therapies2662-76712021-09-0121111410.1186/s12906-021-03409-9Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosisJuan Nie0Yanlu Liu1Chaoyue Sun2Jingna Zheng3Baoyi Chen4Jianyi Zhuo5Ziren Su6Xiaoping Lai7Jiannan Chen8Jibiao Zheng9Yucui Li10School of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine2nd Clinical Hospital of Guangzhou University of Chinese MedicineSchool of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineSchool of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineSchool of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineSchool of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineSchool of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineSchool of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineDepartment of Pharmacy, Central People’s Hospital of ZhanjiangSchool of Pharmaceutical Sciences, Guangzhou University of Chinese MedicineAbstract Background As a prevalent type of cryptogenic fibrotic disease with high mortality, idiopathic pulmonary fibrosis (IPF) still lacks effective therapeutic drugs. The compounds extracted from buds and flowers of Chrysanthemum indicum Linné with supercritical-carbon dioxide fluid (CISCFE) has been confirmed to have antioxidant, anti-inflammatory, and lung-protective effects. This paper aimed to clarify whether CISCFE could treat IPF induced by bleomycin (BLM) and elucidate the related mechanisms. Methods Rats (Sprague-Dawley, male) were separated into the following groups: normal, model, pirfenidone (50 mg/kg), CISCFE-L, −M, and -H (240, 360, and 480 mg/kg/d, i.g., respectively, for 4 weeks). Rats were given BLM (5 mg/kg) via intratracheal installation to establish the IPF model. A549 and MRC-5 cells were stimulated by Wnt-1 to establish a cell model and then treated with CISCFE. Haematoxylin-eosin (H&E) and Masson staining were employed to observe lesions in the lung tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB) were performed to observe changes in genes and proteins connected with the Wnt/β-catenin pathway. Results CISCFE inhibited the proliferation of MRC-5 cells (IC50: 2.723 ± 0.488 μg/mL) and A549 cells (IC50: 2.235 ± 0.229 μg/mL). In rats, A549 cells, and MRC-5 cells, BLM and Wnt-1 obviously induced the protein expression of α-smooth muscle actin (α-SMA), vimentin, type I collagen (collagen-I), and Nu-β-catenin. The mRNA levels of matrix metalloproteinase-3 (MMP-3) and − 9 (MMP-9), two enzymes that degrade and reshape the extracellular matrix (ECM) were also increased while those of tissue inhibitor of metalloproteinase 1 (TIMP-1) were decreased. However, CISCFE reversed the effects of BLM and Wnt-1 on the expression pattern of these proteins and genes. Conclusion These findings showed that CISCFE could inhibit IPF development by activating the Wnt/β-catenin pathway and may serve as a treatment for IPF after further investigation.https://doi.org/10.1186/s12906-021-03409-9Chrysanthemum indicum LinnéSupercritical carbon dioxide extractionIdiopathic pulmonary fibrosisWnt/β-catenin signalling pathway |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Nie Yanlu Liu Chaoyue Sun Jingna Zheng Baoyi Chen Jianyi Zhuo Ziren Su Xiaoping Lai Jiannan Chen Jibiao Zheng Yucui Li |
spellingShingle |
Juan Nie Yanlu Liu Chaoyue Sun Jingna Zheng Baoyi Chen Jianyi Zhuo Ziren Su Xiaoping Lai Jiannan Chen Jibiao Zheng Yucui Li Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosis BMC Complementary Medicine and Therapies Chrysanthemum indicum Linné Supercritical carbon dioxide extraction Idiopathic pulmonary fibrosis Wnt/β-catenin signalling pathway |
author_facet |
Juan Nie Yanlu Liu Chaoyue Sun Jingna Zheng Baoyi Chen Jianyi Zhuo Ziren Su Xiaoping Lai Jiannan Chen Jibiao Zheng Yucui Li |
author_sort |
Juan Nie |
title |
Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosis |
title_short |
Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosis |
title_full |
Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosis |
title_fullStr |
Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosis |
title_full_unstemmed |
Effect of supercritical carbon dioxide fluid extract from Chrysanthemum indicum Linné on bleomycin-induced pulmonary fibrosis |
title_sort |
effect of supercritical carbon dioxide fluid extract from chrysanthemum indicum linné on bleomycin-induced pulmonary fibrosis |
publisher |
BMC |
series |
BMC Complementary Medicine and Therapies |
issn |
2662-7671 |
publishDate |
2021-09-01 |
description |
Abstract Background As a prevalent type of cryptogenic fibrotic disease with high mortality, idiopathic pulmonary fibrosis (IPF) still lacks effective therapeutic drugs. The compounds extracted from buds and flowers of Chrysanthemum indicum Linné with supercritical-carbon dioxide fluid (CISCFE) has been confirmed to have antioxidant, anti-inflammatory, and lung-protective effects. This paper aimed to clarify whether CISCFE could treat IPF induced by bleomycin (BLM) and elucidate the related mechanisms. Methods Rats (Sprague-Dawley, male) were separated into the following groups: normal, model, pirfenidone (50 mg/kg), CISCFE-L, −M, and -H (240, 360, and 480 mg/kg/d, i.g., respectively, for 4 weeks). Rats were given BLM (5 mg/kg) via intratracheal installation to establish the IPF model. A549 and MRC-5 cells were stimulated by Wnt-1 to establish a cell model and then treated with CISCFE. Haematoxylin-eosin (H&E) and Masson staining were employed to observe lesions in the lung tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB) were performed to observe changes in genes and proteins connected with the Wnt/β-catenin pathway. Results CISCFE inhibited the proliferation of MRC-5 cells (IC50: 2.723 ± 0.488 μg/mL) and A549 cells (IC50: 2.235 ± 0.229 μg/mL). In rats, A549 cells, and MRC-5 cells, BLM and Wnt-1 obviously induced the protein expression of α-smooth muscle actin (α-SMA), vimentin, type I collagen (collagen-I), and Nu-β-catenin. The mRNA levels of matrix metalloproteinase-3 (MMP-3) and − 9 (MMP-9), two enzymes that degrade and reshape the extracellular matrix (ECM) were also increased while those of tissue inhibitor of metalloproteinase 1 (TIMP-1) were decreased. However, CISCFE reversed the effects of BLM and Wnt-1 on the expression pattern of these proteins and genes. Conclusion These findings showed that CISCFE could inhibit IPF development by activating the Wnt/β-catenin pathway and may serve as a treatment for IPF after further investigation. |
topic |
Chrysanthemum indicum Linné Supercritical carbon dioxide extraction Idiopathic pulmonary fibrosis Wnt/β-catenin signalling pathway |
url |
https://doi.org/10.1186/s12906-021-03409-9 |
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