Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA
Abstract Objective We have previously demonstrated that unencapsulated Enterococcus faecalis cps2 inhibits biofilm formation of Candida albicans, a fungus commonly found with E. faecalis in periapical lesion. In this study, we compared encapsulated and unencapsulated E. faecalis cps2 strains relatio...
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doaj-5305b30d3a8643c1b7299dff14f8a8002020-11-25T01:30:51ZengBMCBMC Research Notes1756-05002017-08-011011610.1186/s13104-017-2740-4Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNABoy M. Bachtiar0Endang W. Bachtiar1Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas IndonesiaDepartment of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas IndonesiaAbstract Objective We have previously demonstrated that unencapsulated Enterococcus faecalis cps2 inhibits biofilm formation of Candida albicans, a fungus commonly found with E. faecalis in periapical lesion. In this study, we compared encapsulated and unencapsulated E. faecalis cps2 strains relationship with osteoblastic (MG-63) cells, whereas E. faecalis ATCC 29212 were used as a reference strain. Results The binding capacity of E. faecalis to MG-63 cells as shown by each tested strain was comparable, but the unencapsulated strain was less invasive compared to the encapsulated and the reference strains. Moreover, quantitative real time-PCR (qPCR) results showed that infecting unencapsulated E. faecalis cps2 is a stronger stimulator for toll like receptor 2 (TLR2) and interleukin-1β (IL-1β) mRNAs, but not for inducible nitric oxide synthase (iNOS) mRNA in osteoblastic cells. In conclusion, the performance of unencapsulated E. faecalis cps2 when the bacterium interacts with osteoblastic cells is quite different from that of encapsulated E. faecalis cps2 and reference strains. It appears that the unencapsulated strain might contribute to the persistence of the periapical inflammatory response, depending on down-regulation of iNOS mRNA expression.http://link.springer.com/article/10.1186/s13104-017-2740-4Encapsulated and unencapsulated E. faecalis cps2MG-63TLR-2IL-1β and iNOS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Boy M. Bachtiar Endang W. Bachtiar |
spellingShingle |
Boy M. Bachtiar Endang W. Bachtiar Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA BMC Research Notes Encapsulated and unencapsulated E. faecalis cps2 MG-63 TLR-2 IL-1β and iNOS |
author_facet |
Boy M. Bachtiar Endang W. Bachtiar |
author_sort |
Boy M. Bachtiar |
title |
Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA |
title_short |
Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA |
title_full |
Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA |
title_fullStr |
Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA |
title_full_unstemmed |
Proinflammatory MG-63 cells response infection with Enterococcus faecalis cps2 evaluated by the expression of TLR-2, IL-1β, and iNOS mRNA |
title_sort |
proinflammatory mg-63 cells response infection with enterococcus faecalis cps2 evaluated by the expression of tlr-2, il-1β, and inos mrna |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2017-08-01 |
description |
Abstract Objective We have previously demonstrated that unencapsulated Enterococcus faecalis cps2 inhibits biofilm formation of Candida albicans, a fungus commonly found with E. faecalis in periapical lesion. In this study, we compared encapsulated and unencapsulated E. faecalis cps2 strains relationship with osteoblastic (MG-63) cells, whereas E. faecalis ATCC 29212 were used as a reference strain. Results The binding capacity of E. faecalis to MG-63 cells as shown by each tested strain was comparable, but the unencapsulated strain was less invasive compared to the encapsulated and the reference strains. Moreover, quantitative real time-PCR (qPCR) results showed that infecting unencapsulated E. faecalis cps2 is a stronger stimulator for toll like receptor 2 (TLR2) and interleukin-1β (IL-1β) mRNAs, but not for inducible nitric oxide synthase (iNOS) mRNA in osteoblastic cells. In conclusion, the performance of unencapsulated E. faecalis cps2 when the bacterium interacts with osteoblastic cells is quite different from that of encapsulated E. faecalis cps2 and reference strains. It appears that the unencapsulated strain might contribute to the persistence of the periapical inflammatory response, depending on down-regulation of iNOS mRNA expression. |
topic |
Encapsulated and unencapsulated E. faecalis cps2 MG-63 TLR-2 IL-1β and iNOS |
url |
http://link.springer.com/article/10.1186/s13104-017-2740-4 |
work_keys_str_mv |
AT boymbachtiar proinflammatorymg63cellsresponseinfectionwithenterococcusfaecaliscps2evaluatedbytheexpressionoftlr2il1bandinosmrna AT endangwbachtiar proinflammatorymg63cellsresponseinfectionwithenterococcusfaecaliscps2evaluatedbytheexpressionoftlr2il1bandinosmrna |
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1725089372017000448 |