Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour
Background: Early detection would improve upper gastrointestinal cancer prognosis. We aimed to identify serum protein biomarker for the detection of early-stage upper gastrointestinal cancer. Methods: We performed a three-tiered study including 2028 participants from three medical centres. First, we...
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Elsevier
2020-01-01
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Series: | EBioMedicine |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396419307765 |
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doaj-530e430b721547a6a5f83d222b8bd3f5 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi-Wei Xu Hao Chen Chao-Qun Hong Ling-Yu Chu Shi-Han Yang Li-Sheng Huang Hong Guo Liu-Yi Chen Can-Tong Liu Xin-Yi Huang Lie-Hao Lin Shu-Lin Chen Zhi-Yong Wu Yu-Hui Peng Li-Yan Xu En-Min Li |
spellingShingle |
Yi-Wei Xu Hao Chen Chao-Qun Hong Ling-Yu Chu Shi-Han Yang Li-Sheng Huang Hong Guo Liu-Yi Chen Can-Tong Liu Xin-Yi Huang Lie-Hao Lin Shu-Lin Chen Zhi-Yong Wu Yu-Hui Peng Li-Yan Xu En-Min Li Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour EBioMedicine |
author_facet |
Yi-Wei Xu Hao Chen Chao-Qun Hong Ling-Yu Chu Shi-Han Yang Li-Sheng Huang Hong Guo Liu-Yi Chen Can-Tong Liu Xin-Yi Huang Lie-Hao Lin Shu-Lin Chen Zhi-Yong Wu Yu-Hui Peng Li-Yan Xu En-Min Li |
author_sort |
Yi-Wei Xu |
title |
Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour |
title_short |
Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour |
title_full |
Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour |
title_fullStr |
Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour |
title_full_unstemmed |
Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour |
title_sort |
serum igfbp-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumour |
publisher |
Elsevier |
series |
EBioMedicine |
issn |
2352-3964 |
publishDate |
2020-01-01 |
description |
Background: Early detection would improve upper gastrointestinal cancer prognosis. We aimed to identify serum protein biomarker for the detection of early-stage upper gastrointestinal cancer. Methods: We performed a three-tiered study including 2028 participants from three medical centres. First, we applied two different antibody arrays to screen candidate serum proteins that increased in 20 patients with oesophageal squamous cell carcinoma (ESCC) compared with 20 normal controls. We then evaluated the selected protein by enzyme-linked immunosorbent assay in 1064 participants including 731 upper gastrointestinal cancer patients (287 ESCCs, 237 oesophagogastric junction adenocarcinomas (EJAs), and 207 stomach cancers) and 333 normal controls. The diagnostic value of the selected protein was finally validated in two independent cohorts of ESCC patients and controls (n=472 and 452, respectively). The receiver operating characteristic was used to calculate diagnostic accuracy. Findings: Serum insulin-like growth factor binding protein-1 (IGFBP-1) identified in both antibody arrays showed significantly elevated levels in upper gastrointestinal cancers, compared with normal controls. Serum IGFBP-1 provided high diagnostic accuracy of early-stage ESCC, EJA, stomach and cancer (areas under the curve: 0·898, 0·936 and 0·864, respectively). This protein maintained diagnostic performance for early-stage ESCC in independent cohorts 1 and 2 (0·849 and 0·911, respectively). Additionally, serum levels of IGFBP-1 dropped significantly after surgical resection of primary tumours, compared with the corresponding pre-operative ESCC samples (p < 0·05). Interpretation: Serum IGFBP-1 represents a promising diagnostic biomarker to detect early-stage upper gastrointestinal cancer. Keywords: Upper gastrointestinal cancer, IGFBP-1, Early diagnosis, Sensitivity, Specificity |
url |
http://www.sciencedirect.com/science/article/pii/S2352396419307765 |
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doaj-530e430b721547a6a5f83d222b8bd3f52020-11-24T23:07:41ZengElsevierEBioMedicine2352-39642020-01-0151Serum IGFBP-1 as a potential biomarker for diagnosis of early-stage upper gastrointestinal tumourYi-Wei Xu0Hao Chen1Chao-Qun Hong2Ling-Yu Chu3Shi-Han Yang4Li-Sheng Huang5Hong Guo6Liu-Yi Chen7Can-Tong Liu8Xin-Yi Huang9Lie-Hao Lin10Shu-Lin Chen11Zhi-Yong Wu12Yu-Hui Peng13Li-Yan Xu14En-Min Li15Department of Clinical Laboratory Medicine, the Cancer Hospital of Shantou University Medical College, Shantou, China; Precision Medicine Research Centre, Shantou University Medical College, Shantou, China; Guangdong Oesophageal Cancer Research Institute, Shantou University Medical College, Shantou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Centre for Cancer Medicine, Sun Yat-sen University Cancer Centre, Guangzhou, ChinaDepartment of Oncological Laboratory Research, The Cancer Hospital of Shantou University Medical College, Shantou, ChinaPrecision Medicine Research Centre, Shantou University Medical College, Shantou, ChinaDepartment of Dermatology and Venereology, Shantou Central Hospital, Shantou, ChinaDepartment of Radiation Oncology, The Cancer Hospital of Shantou University Medical College, Shantou, ChinaDepartment of Radiation Oncology, The Cancer Hospital of Shantou University Medical College, Shantou, ChinaDepartment of Clinical Laboratory Medicine, the Cancer Hospital of Shantou University Medical College, Shantou, ChinaDepartment of Clinical Laboratory Medicine, the Cancer Hospital of Shantou University Medical College, Shantou, China; Precision Medicine Research Centre, Shantou University Medical College, Shantou, ChinaPrecision Medicine Research Centre, Shantou University Medical College, Shantou, ChinaDepartment of surgery, Shantou Nan'ao People's Hospital, Shantou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Centre for Cancer Medicine, Sun Yat-sen University Cancer Centre, Guangzhou, ChinaDepartment of Surgical Oncology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou, China; Corresponding authors.Department of Clinical Laboratory Medicine, the Cancer Hospital of Shantou University Medical College, Shantou, China; Precision Medicine Research Centre, Shantou University Medical College, Shantou, China; Guangdong Oesophageal Cancer Research Institute, Shantou University Medical College, Shantou, China; Corresponding author at: Department of Clinical Laboratory Medicine, the Cancer Hospital of Shantou University Medical College, Shantou, China.Institute of Oncologic Pathology, Shantou University Medical College, Shantou, China; Corresponding authors.Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou, China; Corresponding authors.Background: Early detection would improve upper gastrointestinal cancer prognosis. We aimed to identify serum protein biomarker for the detection of early-stage upper gastrointestinal cancer. Methods: We performed a three-tiered study including 2028 participants from three medical centres. First, we applied two different antibody arrays to screen candidate serum proteins that increased in 20 patients with oesophageal squamous cell carcinoma (ESCC) compared with 20 normal controls. We then evaluated the selected protein by enzyme-linked immunosorbent assay in 1064 participants including 731 upper gastrointestinal cancer patients (287 ESCCs, 237 oesophagogastric junction adenocarcinomas (EJAs), and 207 stomach cancers) and 333 normal controls. The diagnostic value of the selected protein was finally validated in two independent cohorts of ESCC patients and controls (n=472 and 452, respectively). The receiver operating characteristic was used to calculate diagnostic accuracy. Findings: Serum insulin-like growth factor binding protein-1 (IGFBP-1) identified in both antibody arrays showed significantly elevated levels in upper gastrointestinal cancers, compared with normal controls. Serum IGFBP-1 provided high diagnostic accuracy of early-stage ESCC, EJA, stomach and cancer (areas under the curve: 0·898, 0·936 and 0·864, respectively). This protein maintained diagnostic performance for early-stage ESCC in independent cohorts 1 and 2 (0·849 and 0·911, respectively). Additionally, serum levels of IGFBP-1 dropped significantly after surgical resection of primary tumours, compared with the corresponding pre-operative ESCC samples (p < 0·05). Interpretation: Serum IGFBP-1 represents a promising diagnostic biomarker to detect early-stage upper gastrointestinal cancer. Keywords: Upper gastrointestinal cancer, IGFBP-1, Early diagnosis, Sensitivity, Specificityhttp://www.sciencedirect.com/science/article/pii/S2352396419307765 |