Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae)
Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of <i>Ginkgo biloba</i>, chloroplast microsatellite primers were developed. Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chl...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2013-07-01
|
Series: | Applications in Plant Sciences |
Subjects: | |
Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300019 |
id |
doaj-530eda43e9f842138aab006db8e457c4 |
---|---|
record_format |
Article |
spelling |
doaj-530eda43e9f842138aab006db8e457c42020-11-24T22:26:29ZengWileyApplications in Plant Sciences2168-04502013-07-0118130001910.3732/apps.1300019Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae)Chun-Xiang Xie0Ming-Shui Zhao1Cheng-Xin Fu2Yun-Peng Zhao3Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology, and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of ChinaAdministration Bureau of Tianmu Mountain National Nature Reserve, Lin'an 311311, People's Republic of ChinaLaboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology, and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of ChinaKey Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology, and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of ChinaPremise of the study: To investigate population genetics, phylogeography, and cultivar origin of <i>Ginkgo biloba</i>, chloroplast microsatellite primers were developed. Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes of <i>G. biloba</i>. Polymorphisms were assessed on four natural populations from the two refugia in China. Eight loci were detected to be polymorphic in these populations. The number of alleles per locus ranged from three to seven, and the unbiased haploid diversity per locus varied from 0.441 to 0.807. Conclusions: For the first time, we developed 21 chloroplast microsatellite markers for <i>G. biloba</i>, including 13 monomorphic and eight polymorphic ones within the assessed natural populations. These markers should provide a powerful tool for the study of genetic variation of both natural and cultivated populations of <i>G. biloba</i>, as well as cultivars.http://www.bioone.org/doi/full/10.3732/apps.1300019chloroplast microsatellitecpSSRgenetic diversityGinkgo bilobaGinkgoaceaegymnospermmolecular marker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chun-Xiang Xie Ming-Shui Zhao Cheng-Xin Fu Yun-Peng Zhao |
spellingShingle |
Chun-Xiang Xie Ming-Shui Zhao Cheng-Xin Fu Yun-Peng Zhao Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae) Applications in Plant Sciences chloroplast microsatellite cpSSR genetic diversity Ginkgo biloba Ginkgoaceae gymnosperm molecular marker |
author_facet |
Chun-Xiang Xie Ming-Shui Zhao Cheng-Xin Fu Yun-Peng Zhao |
author_sort |
Chun-Xiang Xie |
title |
Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae) |
title_short |
Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae) |
title_full |
Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae) |
title_fullStr |
Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae) |
title_full_unstemmed |
Development of the First Chloroplast Microsatellite Loci in <i>Ginkgo biloba</i> (Ginkgoaceae) |
title_sort |
development of the first chloroplast microsatellite loci in <i>ginkgo biloba</i> (ginkgoaceae) |
publisher |
Wiley |
series |
Applications in Plant Sciences |
issn |
2168-0450 |
publishDate |
2013-07-01 |
description |
Premise of the study: To investigate population genetics, phylogeography, and cultivar origin of <i>Ginkgo biloba</i>, chloroplast microsatellite primers were developed.
Methods and Results: Twenty-one chloroplast microsatellite markers were identified referring to the two published chloroplast genomes of <i>G. biloba</i>. Polymorphisms were assessed on four natural populations from the two refugia in China. Eight loci were detected to be polymorphic in these populations. The number of alleles per locus ranged from three to seven, and the unbiased haploid diversity per locus varied from 0.441 to 0.807.
Conclusions: For the first time, we developed 21 chloroplast microsatellite markers for <i>G. biloba</i>, including 13 monomorphic and eight polymorphic ones within the assessed natural populations. These markers should provide a powerful tool for the study of genetic variation of both natural and cultivated populations of <i>G. biloba</i>, as well as cultivars. |
topic |
chloroplast microsatellite cpSSR genetic diversity Ginkgo biloba Ginkgoaceae gymnosperm molecular marker |
url |
http://www.bioone.org/doi/full/10.3732/apps.1300019 |
work_keys_str_mv |
AT chunxiangxie developmentofthefirstchloroplastmicrosatellitelociiniginkgobilobaiginkgoaceae AT mingshuizhao developmentofthefirstchloroplastmicrosatellitelociiniginkgobilobaiginkgoaceae AT chengxinfu developmentofthefirstchloroplastmicrosatellitelociiniginkgobilobaiginkgoaceae AT yunpengzhao developmentofthefirstchloroplastmicrosatellitelociiniginkgobilobaiginkgoaceae |
_version_ |
1725753403970158592 |