Implications of targeted next-generation sequencing for bladder cancer: report of four cases
Abstract Background Bladder cancer is considered heterogeneous diseases with two major subgroups: non-muscle- invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). It is a major healthcare problem, and it is one of the leading causes of mortality worldwide. Genetic mutations are...
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doaj-531b40081975468d9ac1d44dd0919be32021-06-27T11:44:48ZengSpringerOpenJournal of Genetic Engineering and Biotechnology2090-59202021-06-011911810.1186/s43141-021-00182-7Implications of targeted next-generation sequencing for bladder cancer: report of four casesMohamed K. Khalifa0Noha M. Bakr1Amal Ramadan2Khaled M. Abd Elwahab3Esam Desoky4Amira M. Nageeb5Menha Swellam6CSO at OmicsenseBiochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research CentreBiochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research CentreUrology Department, Zagazig UniversityUrology Department, Zagazig UniversityBiochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research CentreBiochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research CentreAbstract Background Bladder cancer is considered heterogeneous diseases with two major subgroups: non-muscle- invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). It is a major healthcare problem, and it is one of the leading causes of mortality worldwide. Genetic mutations are not only a cause for carcinogenesis but are also a way for treatment strategy. The present study aimed to investigate breast cancer (BRCA genes) tumor suppressor gene mutations in bladder cancer tissue and combined blood samples for patients who developed secondary tumor after or during trimodal therapy. Fresh tissue samples and their matched blood samples were collected from four patients with bladder cancer. The objective regions for the examined genes (BRCA1 and BRCA2) were sequenced using next-generation sequencing (NGS); generated BAM files were uploaded to the cloud-based Ionreporter server, and the Oncomine BRCA-specific plugin was used to analyze the paired normal and tumor sample for each patient using the default plugin parameters. Results Intronic BRCA1 mutation c.5050-104 C >T was reported among the four investigated bladder cancer patients, and three somatic mutations were reported as follows: two of them were found to be benign rs1064793056 and rs28897679 on the Clinivar database and one nonsense pathogenic variant rs80357006. BRCA 2 gene mutation reported an exonic synonymous mutation rs397507876 in the tissue and germline DNA. Patients were treated with trimodal; however, three bladder cancer patients who reported BRCA mutations developed secondary tumors. Conclusion Identification of mutational BRCA changes in bladder cancer is a promising marker for better treatment strategy. Further studies are encouraged on a large cohort of bladder cancer patients to confirm our findings.https://doi.org/10.1186/s43141-021-00182-7Bladder cancerSomatic mutationGermline mutationBRCA genesNext-generation sequencingCase report |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed K. Khalifa Noha M. Bakr Amal Ramadan Khaled M. Abd Elwahab Esam Desoky Amira M. Nageeb Menha Swellam |
spellingShingle |
Mohamed K. Khalifa Noha M. Bakr Amal Ramadan Khaled M. Abd Elwahab Esam Desoky Amira M. Nageeb Menha Swellam Implications of targeted next-generation sequencing for bladder cancer: report of four cases Journal of Genetic Engineering and Biotechnology Bladder cancer Somatic mutation Germline mutation BRCA genes Next-generation sequencing Case report |
author_facet |
Mohamed K. Khalifa Noha M. Bakr Amal Ramadan Khaled M. Abd Elwahab Esam Desoky Amira M. Nageeb Menha Swellam |
author_sort |
Mohamed K. Khalifa |
title |
Implications of targeted next-generation sequencing for bladder cancer: report of four cases |
title_short |
Implications of targeted next-generation sequencing for bladder cancer: report of four cases |
title_full |
Implications of targeted next-generation sequencing for bladder cancer: report of four cases |
title_fullStr |
Implications of targeted next-generation sequencing for bladder cancer: report of four cases |
title_full_unstemmed |
Implications of targeted next-generation sequencing for bladder cancer: report of four cases |
title_sort |
implications of targeted next-generation sequencing for bladder cancer: report of four cases |
publisher |
SpringerOpen |
series |
Journal of Genetic Engineering and Biotechnology |
issn |
2090-5920 |
publishDate |
2021-06-01 |
description |
Abstract Background Bladder cancer is considered heterogeneous diseases with two major subgroups: non-muscle- invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). It is a major healthcare problem, and it is one of the leading causes of mortality worldwide. Genetic mutations are not only a cause for carcinogenesis but are also a way for treatment strategy. The present study aimed to investigate breast cancer (BRCA genes) tumor suppressor gene mutations in bladder cancer tissue and combined blood samples for patients who developed secondary tumor after or during trimodal therapy. Fresh tissue samples and their matched blood samples were collected from four patients with bladder cancer. The objective regions for the examined genes (BRCA1 and BRCA2) were sequenced using next-generation sequencing (NGS); generated BAM files were uploaded to the cloud-based Ionreporter server, and the Oncomine BRCA-specific plugin was used to analyze the paired normal and tumor sample for each patient using the default plugin parameters. Results Intronic BRCA1 mutation c.5050-104 C >T was reported among the four investigated bladder cancer patients, and three somatic mutations were reported as follows: two of them were found to be benign rs1064793056 and rs28897679 on the Clinivar database and one nonsense pathogenic variant rs80357006. BRCA 2 gene mutation reported an exonic synonymous mutation rs397507876 in the tissue and germline DNA. Patients were treated with trimodal; however, three bladder cancer patients who reported BRCA mutations developed secondary tumors. Conclusion Identification of mutational BRCA changes in bladder cancer is a promising marker for better treatment strategy. Further studies are encouraged on a large cohort of bladder cancer patients to confirm our findings. |
topic |
Bladder cancer Somatic mutation Germline mutation BRCA genes Next-generation sequencing Case report |
url |
https://doi.org/10.1186/s43141-021-00182-7 |
work_keys_str_mv |
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