Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media
The aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or...
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Instituto Oswaldo Cruz, Ministério da Saúde
2010-08-01
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doaj-533937da6e924a75b6c7edfd48fff94f2020-11-24T23:19:55ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602010-08-01105571771910.1590/S0074-02762010000500020Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture mediaAngela de OliveiraAdivaldo Henrique FonsecaCatia Marques da CostaElenice MantovaniNatalino Hajime YoshinariThe aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or Petri dishes. For each medium, the initial spirochete concentration was standardized to 10³ spirochets/mL. The following culture media were suitable to grow B. garinii: Barbour-Stoenner-Kelly, brain heart infusion and PMR. Growth was minimal at six weeks post-inoculation and maximum spirochete density was observed between 9-12 weeks. Often, the cultures developed cysts of different sizes, isolated or in groups, with a spiraled portion of variable sizes, mainly in unfavorable culture media. Brazilian Lyme disease-like illness, also known as Baggio-Yoshinari syndrome (BYS), is a new and interesting emerging tick-borne disease, caused by Borrelia burgdorferi sensu lato spirochetes, only during its cystic forms. It has been assumed that the peculiar clinical and laboratory features of BYS are consequential to the absence of a human sucker Ixodes ricinus complex tick at risk areas in Brazil, supporting the concept that the borrelia phenotypic expression pattern is modified as it is transmitted through the host.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500020culture mediaBorrelia gariniispirochaetaceaekinetic growthcysts |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Angela de Oliveira Adivaldo Henrique Fonseca Catia Marques da Costa Elenice Mantovani Natalino Hajime Yoshinari |
spellingShingle |
Angela de Oliveira Adivaldo Henrique Fonseca Catia Marques da Costa Elenice Mantovani Natalino Hajime Yoshinari Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media Memórias do Instituto Oswaldo Cruz. culture media Borrelia garinii spirochaetaceae kinetic growth cysts |
author_facet |
Angela de Oliveira Adivaldo Henrique Fonseca Catia Marques da Costa Elenice Mantovani Natalino Hajime Yoshinari |
author_sort |
Angela de Oliveira |
title |
Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media |
title_short |
Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media |
title_full |
Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media |
title_fullStr |
Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media |
title_full_unstemmed |
Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media |
title_sort |
growth, cysts and kinetics of borrelia garinii (spirochaetales: spirochaetacea) in different culture media |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2010-08-01 |
description |
The aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or Petri dishes. For each medium, the initial spirochete concentration was standardized to 10³ spirochets/mL. The following culture media were suitable to grow B. garinii: Barbour-Stoenner-Kelly, brain heart infusion and PMR. Growth was minimal at six weeks post-inoculation and maximum spirochete density was observed between 9-12 weeks. Often, the cultures developed cysts of different sizes, isolated or in groups, with a spiraled portion of variable sizes, mainly in unfavorable culture media. Brazilian Lyme disease-like illness, also known as Baggio-Yoshinari syndrome (BYS), is a new and interesting emerging tick-borne disease, caused by Borrelia burgdorferi sensu lato spirochetes, only during its cystic forms. It has been assumed that the peculiar clinical and laboratory features of BYS are consequential to the absence of a human sucker Ixodes ricinus complex tick at risk areas in Brazil, supporting the concept that the borrelia phenotypic expression pattern is modified as it is transmitted through the host. |
topic |
culture media Borrelia garinii spirochaetaceae kinetic growth cysts |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500020 |
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