Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants
Summary: Lipids are major components of membranes with pleiotropic roles and interconnected metabolism, so experimentally addressing the primary function of individual lipid species in vivo can be difficult. Genetic approaches are particularly challenging to interpret due to compensatory mechanisms...
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2021-09-01
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doaj-534564521a9a48a99f56229df6a2889b2021-09-19T04:59:58ZengElsevierSTAR Protocols2666-16672021-09-0123100626Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutantsMatthieu Pierre Platre0Yvon Jaillais1Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, 69342 Lyon, France; Corresponding authorLaboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, 69342 Lyon, France; Corresponding authorSummary: Lipids are major components of membranes with pleiotropic roles and interconnected metabolism, so experimentally addressing the primary function of individual lipid species in vivo can be difficult. Genetic approaches are particularly challenging to interpret due to compensatory mechanisms and indirect effects. Here, we describe a fast inducible approach to complement the phenotypes of Arabidopsis lipid mutants through exogenous treatment with the depleted lipid, followed by live confocal imaging to observe genetically encoded lipid sensors in wild-type and mutant root tissues.For complete details on the use and execution of this protocol, please refer to Platre et al. (2018).http://www.sciencedirect.com/science/article/pii/S2666166721003336Cell MembraneMicroscopyModel OrganismsPlant sciencesMolecular/Chemical Probes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthieu Pierre Platre Yvon Jaillais |
spellingShingle |
Matthieu Pierre Platre Yvon Jaillais Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants STAR Protocols Cell Membrane Microscopy Model Organisms Plant sciences Molecular/Chemical Probes |
author_facet |
Matthieu Pierre Platre Yvon Jaillais |
author_sort |
Matthieu Pierre Platre |
title |
Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants |
title_short |
Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants |
title_full |
Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants |
title_fullStr |
Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants |
title_full_unstemmed |
Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants |
title_sort |
exogenous treatment of arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants |
publisher |
Elsevier |
series |
STAR Protocols |
issn |
2666-1667 |
publishDate |
2021-09-01 |
description |
Summary: Lipids are major components of membranes with pleiotropic roles and interconnected metabolism, so experimentally addressing the primary function of individual lipid species in vivo can be difficult. Genetic approaches are particularly challenging to interpret due to compensatory mechanisms and indirect effects. Here, we describe a fast inducible approach to complement the phenotypes of Arabidopsis lipid mutants through exogenous treatment with the depleted lipid, followed by live confocal imaging to observe genetically encoded lipid sensors in wild-type and mutant root tissues.For complete details on the use and execution of this protocol, please refer to Platre et al. (2018). |
topic |
Cell Membrane Microscopy Model Organisms Plant sciences Molecular/Chemical Probes |
url |
http://www.sciencedirect.com/science/article/pii/S2666166721003336 |
work_keys_str_mv |
AT matthieupierreplatre exogenoustreatmentofarabidopsisseedlingswithlysophospholipidsfortheinduciblecomplementationoflipidmutants AT yvonjaillais exogenoustreatmentofarabidopsisseedlingswithlysophospholipidsfortheinduciblecomplementationoflipidmutants |
_version_ |
1717376204771688448 |