Comparative study of I/D polymorphism of ace gene in diabetes type-2 patients and control group in unrelated Gujarati population

• Main Authors: Doshi Darshan D., Shah Druti B., Singh Krishna M., Patel Rajesh K.
• Format: Article
• Language: English
• Published: Serbian Genetics Society 2015-01-01
• Series: Genetika
• Subjects: I/D polymorphism; ACE gene; DNA; PCR; Genotype and gene frequencies
• Online Access: http://www.doiserbia.nb.rs/img/doi/0534-0012/2015/0534-00121503013D.pdf

Many of the previous studies indicated that the I/D polymorphism of ACE gene is associated with diabetes type-2. To validate the association of I/D polymorphism in ACE gene, a study was designed in non-diabetic (normal) and diabetic type-2 patients of unrelated Gujarati population. The random blood samples from 36 normal and 36 diabetic type -2 patients of above 45 years were collected for the studies. DNA was extracted from blood samples for PCR by using ACE specific primers. The gene and genotype frequencies were estimated for different alleles observed in diabetic as well as in normal healthy persons. In present study, all three genotypes that is, I/I (477bp), I/D (477/190bp), D/D (190bp) were observed in samples from normal and diabetic patients. Among all genotypes ID (58.3%) has maximum genotypic frequency in diabetic than Non diabetic individuals (44.4%), frequency of II (27.7%) is more in Non diabetic individuals than Diabetic individuals (19.4%) and genotypic frequency of DD (27.7%) is more in Non diabetic than Diabetic individuals (22.22%). The results were not in agreement with so many previous studies. However, recent findings of other studies conducted in different ethnic groups are similar to our findings which do not support that I/D polymorphism are associated with type-2 diabetes.