IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLS
Nonylphenol (NP) is an important environmental toxicant and potential endocrine disrupting chemical. It is also the primary final biodegradation product of nonylphenol polyethoxylate, a non-ionic surfactant that is frequently incorporated into detergent formulation. Several studies have reported adv...
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Slovak University of Agriculture
2015-02-01
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doaj-53546001bfae45abbc936d479033c39a2020-11-25T00:35:58ZengSlovak University of AgricultureJournal of Microbiology, Biotechnology and Food Sciences1338-51782015-02-01vol. 4special issue 2 (Biotechnology)42443719-1IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLSTomáš JamborJana LukáčováEva TvrdáZuzana KňažickáZsolt ForgácsNorbert LukáčNonylphenol (NP) is an important environmental toxicant and potential endocrine disrupting chemical. It is also the primary final biodegradation product of nonylphenol polyethoxylate, a non-ionic surfactant that is frequently incorporated into detergent formulation. Several studies have reported adverse effects of NP on the male reproductive system, including hormonal changes and testicular abnormalities. However, its mechanisms are not clearly understood. In the present study, experiments were performed to examine the effects of 4-nonylphenol on the testosterone production in mice Leydig cells. Interstitial (Leydig) cells were cultured with addition of 0.04, 0.2, 1.0, 2.5 and 5 μg/mL of 4-nonylphenol and compared with control. Concentrations of testosterone in the media were determined using enzyme linked immunosorbent assay (ELISA). Hormone production significantly (P<0.05) increased at 1.0, 2.5 and 5.0 μg/mL of 4-nonylphenol concentrations. Results of the study indicated dose-dependent increases in testosterone production of interstitial (Leydig) cells following a 48 h in vitro 4-nonylphenol exposure. Further investigations are required to establish the biological significance and possible reproductive implications.http://www.jmbfs.org/wp-content/uploads/2015/02/Jambor.pdfLeydig cellsnonylphenoltestosteroneELISA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomáš Jambor Jana Lukáčová Eva Tvrdá Zuzana Kňažická Zsolt Forgács Norbert Lukáč |
spellingShingle |
Tomáš Jambor Jana Lukáčová Eva Tvrdá Zuzana Kňažická Zsolt Forgács Norbert Lukáč IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLS Journal of Microbiology, Biotechnology and Food Sciences Leydig cells nonylphenol testosterone ELISA |
author_facet |
Tomáš Jambor Jana Lukáčová Eva Tvrdá Zuzana Kňažická Zsolt Forgács Norbert Lukáč |
author_sort |
Tomáš Jambor |
title |
IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLS |
title_short |
IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLS |
title_full |
IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLS |
title_fullStr |
IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLS |
title_full_unstemmed |
IMPACT OF 4-NONYLPHENOL ON TESTOSTERONE PRODUCTION IN MICE LEYDIG CELLS |
title_sort |
impact of 4-nonylphenol on testosterone production in mice leydig cells |
publisher |
Slovak University of Agriculture |
series |
Journal of Microbiology, Biotechnology and Food Sciences |
issn |
1338-5178 |
publishDate |
2015-02-01 |
description |
Nonylphenol (NP) is an important environmental toxicant and potential endocrine disrupting chemical. It is also the primary final biodegradation product of nonylphenol polyethoxylate, a non-ionic surfactant that is frequently incorporated into detergent formulation. Several studies have reported adverse effects of NP on the male reproductive system, including hormonal changes and testicular abnormalities. However, its mechanisms are not clearly understood. In the present study, experiments were performed to examine the effects of 4-nonylphenol on the testosterone production in mice Leydig cells. Interstitial (Leydig) cells were cultured with addition of 0.04, 0.2, 1.0, 2.5 and 5 μg/mL of 4-nonylphenol and compared with control. Concentrations of testosterone in the media were determined using enzyme linked immunosorbent assay (ELISA). Hormone production significantly (P<0.05) increased at 1.0, 2.5 and 5.0 μg/mL of 4-nonylphenol concentrations. Results of the study indicated dose-dependent increases in testosterone production of interstitial (Leydig) cells following a 48 h in vitro 4-nonylphenol exposure. Further investigations are required to establish the biological significance and possible reproductive implications. |
topic |
Leydig cells nonylphenol testosterone ELISA |
url |
http://www.jmbfs.org/wp-content/uploads/2015/02/Jambor.pdf |
work_keys_str_mv |
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