Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier
The aim of this study was to compare the effect of new synthetic 4-tiazolidinone derivatives (compounds 3882, 3288 and 3833) and doxorubicin (positive control) in free form and in their complexes with synthetic polyethyleneglycol-containing nanoscale polymeric carrier on the biochemical indicators o...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2015-04-01
|
Series: | Ukrainian Biochemical Journal |
Subjects: | |
Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Kobylinska_2_15.pdf |
id |
doaj-535dc5db2a9149439e6172359ecad216 |
---|---|
record_format |
Article |
spelling |
doaj-535dc5db2a9149439e6172359ecad2162020-11-25T02:43:20ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Ukrainian Biochemical Journal2409-49432413-50032015-04-0187212213210.15407/ubj87.02.122Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrierL. I. Kоbylinska0D. Ya. Havrylyuk1А. О. Ryabtseva2N. E. Mitina3О. S. Zаichenko4R. B. Lesyk5B. S. Zіmenkovsky6R. S. Stoika7Danylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineLviv Polytechnic National University, UkraineLviv Polytechnic National University, UkraineLviv Polytechnic National University, UkraineDanylo Halytsky Lviv National Medical University, UkraineDanylo Halytsky Lviv National Medical University, UkraineІnstitute of Cell Biology, National Academy of Sciences of Ukraine, LvivThe aim of this study was to compare the effect of new synthetic 4-tiazolidinone derivatives (compounds 3882, 3288 and 3833) and doxorubicin (positive control) in free form and in their complexes with synthetic polyethyleneglycol-containing nanoscale polymeric carrier on the biochemical indicators of hepatotoxicity in blood serum of rats. The activity of enzymes considered as the markers of hepatotoxicity, as well as the concentration of total protein, urea and creatinine were measured in blood serum of rats. It was found that after injection of investigated compounds the activities of alanine aminotransferase, alkaline phosphatase and α-amylase increased in comparison to control. Doxorubicin injection was accompanied by 4-fold increase in the activity of γ-glutamyltransferase, and injection of compound 3833 led to 2.5-fold elevation of the activity of this enzyme. Complexation of these аntineoplastic derivatives with a synthetic nanocarrier lowered the activity of the investigated enzymes substantially if compared to the effect of these compounds in free form. The most evident decrease was measured for α-amylase, γ-glutamyltransferase and lactate dehydrogenase activities. The normalization of concentrations of total protein, urea and creatinine in blood serum of rats treated with complexes of the studied compounds with a polymeric carrier comparing with their introduction in free form was also detected. Thus, the immobilization by novel polymeric carrier of anticancer drugs possessing high general toxicity in the treated organism mitigates their toxic effect, which is evident as normalization of specific biochemical indicators of the hepatodestructive effects of the anticancer drugs.http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Kobylinska_2_15.pdf4-thiazolidinone derivativesblood serumdoxorubicinenzyme activityhepatotoxicitypolymeric nanocarrierrats |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
L. I. Kоbylinska D. Ya. Havrylyuk А. О. Ryabtseva N. E. Mitina О. S. Zаichenko R. B. Lesyk B. S. Zіmenkovsky R. S. Stoika |
spellingShingle |
L. I. Kоbylinska D. Ya. Havrylyuk А. О. Ryabtseva N. E. Mitina О. S. Zаichenko R. B. Lesyk B. S. Zіmenkovsky R. S. Stoika Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier Ukrainian Biochemical Journal 4-thiazolidinone derivatives blood serum doxorubicin enzyme activity hepatotoxicity polymeric nanocarrier rats |
author_facet |
L. I. Kоbylinska D. Ya. Havrylyuk А. О. Ryabtseva N. E. Mitina О. S. Zаichenko R. B. Lesyk B. S. Zіmenkovsky R. S. Stoika |
author_sort |
L. I. Kоbylinska |
title |
Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier |
title_short |
Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier |
title_full |
Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier |
title_fullStr |
Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier |
title_full_unstemmed |
Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier |
title_sort |
biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier |
publisher |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. |
series |
Ukrainian Biochemical Journal |
issn |
2409-4943 2413-5003 |
publishDate |
2015-04-01 |
description |
The aim of this study was to compare the effect of new synthetic 4-tiazolidinone derivatives (compounds 3882, 3288 and 3833) and doxorubicin (positive control) in free form and in their complexes with synthetic polyethyleneglycol-containing nanoscale polymeric carrier on the biochemical indicators of hepatotoxicity in blood serum of rats. The activity of enzymes considered as the markers of hepatotoxicity, as well as the concentration of total protein, urea and creatinine were measured in blood serum of rats. It was found that after injection of investigated compounds the activities of alanine aminotransferase, alkaline phosphatase and α-amylase increased in comparison to control. Doxorubicin injection was accompanied by 4-fold increase in the activity of γ-glutamyltransferase, and injection of compound 3833 led to 2.5-fold elevation of the activity of this enzyme. Complexation of these аntineoplastic derivatives with a synthetic nanocarrier lowered the activity of the investigated enzymes substantially if compared to the effect of these compounds in free form. The most evident decrease was measured for α-amylase, γ-glutamyltransferase and lactate dehydrogenase activities. The normalization of concentrations of total protein, urea and creatinine in blood serum of rats treated with complexes of the studied compounds with a polymeric carrier comparing with their introduction in free form was also detected. Thus, the immobilization by novel polymeric carrier of anticancer drugs possessing high general toxicity in the treated organism mitigates their toxic effect, which is evident as normalization of specific biochemical indicators of the hepatodestructive effects of the anticancer drugs. |
topic |
4-thiazolidinone derivatives blood serum doxorubicin enzyme activity hepatotoxicity polymeric nanocarrier rats |
url |
http://ukrbiochemjournal.org/wp-content/uploads/2015/05/Kobylinska_2_15.pdf |
work_keys_str_mv |
AT likobylinska biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier AT dyahavrylyuk biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier AT aoryabtseva biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier AT nemitina biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier AT oszaichenko biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier AT rblesyk biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier AT bszímenkovsky biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier AT rsstoika biochemicalindicatorsofhepatotoxicityinbloodserumofratsundertheeffectofnovel4thiazolidinonederivativesanddoxorubicinandtheircomplexeswithpolyethyleneglycolcontainingnanoscalepolymericcarrier |
_version_ |
1724770026052911104 |