Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence
Background: Recent studies have supported the beneficial effects of a short abstinence period on sperm parameters. The aim of this study was to assess sperm motility, morphology and DNA fragmentation before and after swim-up of a second ejaculate obtained after a short abstinence period in normozoos...
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doaj-537705a8d60d48389af4d23eedfb40502020-11-25T02:26:48ZengMDPI AGJournal of Clinical Medicine2077-03832020-04-0191029102910.3390/jcm9041029Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of AbstinenceClaudio Manna0Federica Barbagallo1Raffaella Manzo2Ashraf Rahman3Davide Francomano4Aldo E. Calogero5Biofertility IVF and Infertility Center, 00198 Rome, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95125 Catania, ItalyBiofertility IVF and Infertility Center, 00198 Rome, ItalyBiofertility IVF and Infertility Center, 00198 Rome, ItalyUnit of Internal Medicine and Endocrinology, Madonna delle Grazie Hospital, Velletri, 75100 Rome, ItalyDepartment of Clinical and Experimental Medicine, University of Catania, 95125 Catania, ItalyBackground: Recent studies have supported the beneficial effects of a short abstinence period on sperm parameters. The aim of this study was to assess sperm motility, morphology and DNA fragmentation before and after swim-up of a second ejaculate obtained after a short abstinence period in normozoospermic men and oligo-astheno-teratozoospermic (OAT) patients. Material and methods: Semen analyses and swim-up preparations of two consecutive semen samples (collected within 1 h) were carried out in 30 normozoospermic and 35 OAT patients enrolled in an assisted reproductive technique (ART) program. Results: Compared to the first ejaculate, the second sample showed a higher percentage of spermatozoa with normal form (<i>p</i> < 0.01) and lower percentage of spermatozoa with DNA fragmentation (<i>p</i> < 0.01) in normozoospermic men, whereas a higher percentage of spermatozoa with progressive motility (<i>p</i> < 0.001) and normal morphology (<i>p</i> < 0.0001) was found in OAT patients. Swim-up separation showed a lower DNA fragmentation rate (<i>p</i> < 0.05) in the second ejaculate in normozoospermic men, whereas the second ejaculate of OAT patents showed an increase in normally-shaped spermatozoa (<i>p</i> < 0.01) and lower percentage of spermatozoa with fragmented DNA (<i>p</i> < 0.001) compared to the first one. Conclusions: Swim-up separation of a second ejaculate collected within 1 h might be suggested for ART procedures, especially in OAT patients.https://www.mdpi.com/2077-0383/9/4/1029sperm morphologysperm motilitysperm DNA fragmentationARTsemen analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Claudio Manna Federica Barbagallo Raffaella Manzo Ashraf Rahman Davide Francomano Aldo E. Calogero |
spellingShingle |
Claudio Manna Federica Barbagallo Raffaella Manzo Ashraf Rahman Davide Francomano Aldo E. Calogero Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence Journal of Clinical Medicine sperm morphology sperm motility sperm DNA fragmentation ART semen analysis |
author_facet |
Claudio Manna Federica Barbagallo Raffaella Manzo Ashraf Rahman Davide Francomano Aldo E. Calogero |
author_sort |
Claudio Manna |
title |
Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence |
title_short |
Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence |
title_full |
Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence |
title_fullStr |
Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence |
title_full_unstemmed |
Sperm Parameters before and after Swim-Up of a Second Ejaculate after a Short Period of Abstinence |
title_sort |
sperm parameters before and after swim-up of a second ejaculate after a short period of abstinence |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-04-01 |
description |
Background: Recent studies have supported the beneficial effects of a short abstinence period on sperm parameters. The aim of this study was to assess sperm motility, morphology and DNA fragmentation before and after swim-up of a second ejaculate obtained after a short abstinence period in normozoospermic men and oligo-astheno-teratozoospermic (OAT) patients. Material and methods: Semen analyses and swim-up preparations of two consecutive semen samples (collected within 1 h) were carried out in 30 normozoospermic and 35 OAT patients enrolled in an assisted reproductive technique (ART) program. Results: Compared to the first ejaculate, the second sample showed a higher percentage of spermatozoa with normal form (<i>p</i> < 0.01) and lower percentage of spermatozoa with DNA fragmentation (<i>p</i> < 0.01) in normozoospermic men, whereas a higher percentage of spermatozoa with progressive motility (<i>p</i> < 0.001) and normal morphology (<i>p</i> < 0.0001) was found in OAT patients. Swim-up separation showed a lower DNA fragmentation rate (<i>p</i> < 0.05) in the second ejaculate in normozoospermic men, whereas the second ejaculate of OAT patents showed an increase in normally-shaped spermatozoa (<i>p</i> < 0.01) and lower percentage of spermatozoa with fragmented DNA (<i>p</i> < 0.001) compared to the first one. Conclusions: Swim-up separation of a second ejaculate collected within 1 h might be suggested for ART procedures, especially in OAT patients. |
topic |
sperm morphology sperm motility sperm DNA fragmentation ART semen analysis |
url |
https://www.mdpi.com/2077-0383/9/4/1029 |
work_keys_str_mv |
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