Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii
Abstract Background The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasi...
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doaj-53802098d15f41d5820641442029b9482020-11-24T21:13:29ZengBMCBMC Genomics1471-21642017-12-0118111210.1186/s12864-017-4362-6Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondiiMusa A. Hassan0Juan J. Vasquez1Chew Guo-Liang2Markus Meissner3T. Nicolai Siegel4Division of Infection and Immunity, The Roslin Institute, University of EdinburghResearch Centre for Infectious Diseases, University of WuerzburgComputational Biology Program, Basic Sciences and Public Health Sciences Division, Fred Hutchinson Cancer Research CentreWellcome Centre for Molecular Parasitology, University of GlasgowResearch Centre for Infectious Diseases, University of WuerzburgAbstract Background The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorous vacuole, the brief entry into the extracellular space is likely to exert enormous stress. Due to its role in cellular stress response, we hypothesize that translational control plays an important role in regulating gene expression in Toxoplasma during the lytic cycle. Unlike transcriptional profiles, insights into genome-wide translational profiles of Toxoplasma gondii are lacking. Methods We have performed genome-wide ribosome profiling, coupled with high throughput RNA sequencing, in intracellular and extracellular Toxoplasma gondii parasites to investigate translational control during the lytic cycle. Results Although differences in transcript abundance were mostly mirrored at the translational level, we observed significant differences in the abundance of ribosome footprints between the two parasite stages. Furthermore, our data suggest that mRNA translation in the parasite is potentially regulated by mRNA secondary structure and upstream open reading frames. Conclusion We show that most of the Toxoplasma genes that are dysregulated during the lytic cycle are translationally regulated.http://link.springer.com/article/10.1186/s12864-017-4362-6Ribosome profilingRNA-sequencingTranslation efficiencyToxoplasma gondiiApicomplexan |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Musa A. Hassan Juan J. Vasquez Chew Guo-Liang Markus Meissner T. Nicolai Siegel |
spellingShingle |
Musa A. Hassan Juan J. Vasquez Chew Guo-Liang Markus Meissner T. Nicolai Siegel Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii BMC Genomics Ribosome profiling RNA-sequencing Translation efficiency Toxoplasma gondii Apicomplexan |
author_facet |
Musa A. Hassan Juan J. Vasquez Chew Guo-Liang Markus Meissner T. Nicolai Siegel |
author_sort |
Musa A. Hassan |
title |
Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
title_short |
Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
title_full |
Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
title_fullStr |
Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
title_full_unstemmed |
Comparative ribosome profiling uncovers a dominant role for translational control in Toxoplasma gondii |
title_sort |
comparative ribosome profiling uncovers a dominant role for translational control in toxoplasma gondii |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2017-12-01 |
description |
Abstract Background The lytic cycle of the protozoan parasite Toxoplasma gondii, which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorous vacuole, the brief entry into the extracellular space is likely to exert enormous stress. Due to its role in cellular stress response, we hypothesize that translational control plays an important role in regulating gene expression in Toxoplasma during the lytic cycle. Unlike transcriptional profiles, insights into genome-wide translational profiles of Toxoplasma gondii are lacking. Methods We have performed genome-wide ribosome profiling, coupled with high throughput RNA sequencing, in intracellular and extracellular Toxoplasma gondii parasites to investigate translational control during the lytic cycle. Results Although differences in transcript abundance were mostly mirrored at the translational level, we observed significant differences in the abundance of ribosome footprints between the two parasite stages. Furthermore, our data suggest that mRNA translation in the parasite is potentially regulated by mRNA secondary structure and upstream open reading frames. Conclusion We show that most of the Toxoplasma genes that are dysregulated during the lytic cycle are translationally regulated. |
topic |
Ribosome profiling RNA-sequencing Translation efficiency Toxoplasma gondii Apicomplexan |
url |
http://link.springer.com/article/10.1186/s12864-017-4362-6 |
work_keys_str_mv |
AT musaahassan comparativeribosomeprofilinguncoversadominantrolefortranslationalcontrolintoxoplasmagondii AT juanjvasquez comparativeribosomeprofilinguncoversadominantrolefortranslationalcontrolintoxoplasmagondii AT chewguoliang comparativeribosomeprofilinguncoversadominantrolefortranslationalcontrolintoxoplasmagondii AT markusmeissner comparativeribosomeprofilinguncoversadominantrolefortranslationalcontrolintoxoplasmagondii AT tnicolaisiegel comparativeribosomeprofilinguncoversadominantrolefortranslationalcontrolintoxoplasmagondii |
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1716748960337494016 |