Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632.
Keratinocytes are the most abundant cell type in the epidermis. They prevent desiccation and provide immunological and barrier defense against potential pathogens such as Staphylococcus aureus and Candida albicans. The study of this first line of immune defense may be hindered by invasive isolation...
Main Authors: | Erik D Anderson, Inka Sastalla, Noah J Earland, Minai Mahnaz, Ian N Moore, Francisco Otaizo-Carrasquero, Timothy G Myers, Christopher A Myles, Sandip K Datta, Ian A Myles |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2018-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC6135349?pdf=render |
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