The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats
Objective: Ischemia/reperfusion (I/R) injury is an important cause of myocardial damage by means of oxidative, inflammatory, and apoptotic mechanisms. The aim of the present study was to examine the potential cardio protective effects of levosimendan in a diabetic rat model of myocardial I/R injury....
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2015-12-01
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doaj-538ce1c9c7c64b508734c9c7f7d613952020-11-24T22:38:48ZengTaylor & Francis GroupLibyan Journal of Medicine1819-63572015-12-011001610.3402/ljm.v10.2926929269The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic ratsHasan Ali Kiraz0Fatih Poyraz1Gülay Kip2Özlem Erdem3Metin Alkan4Mustafa Arslan5Abdullah Özer6Volkan Şivgin7Faruk Metin Çomu8 Department of Anaesthesiology and Reanimation, Onsekiz Mart University Medical Faculty, Canakkale, Turkey Department of Cardiology, Kirikkale University Medical Faculty, Afyonkarahisar, Turkey Department of Paediatric Dentistry (Anaesthesiology and Reanimation specialist), Gazi University Dentistry Faculty, Ankara, Turkey Department of Pathology, Gazi University Medical Faculty, Ankara, Turkey Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, Turkey Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey Department of Physiology, Kirikkale University Medical Faculty, Afyonkarahisar, TurkeyObjective: Ischemia/reperfusion (I/R) injury is an important cause of myocardial damage by means of oxidative, inflammatory, and apoptotic mechanisms. The aim of the present study was to examine the potential cardio protective effects of levosimendan in a diabetic rat model of myocardial I/R injury. Methods: A total of 18 streptozotocin-induced diabetic Wistar Albino rats (55 mg/kg) were randomly divided into three equal groups as follows: the diabetic I/R group (DIR) in which myocardial I/R was induced following left thoracotomy, by ligating the left anterior descending coronary artery for 60 min, followed by 2 h of reperfusion; the diabetic I/R levosimendan group (DIRL), which underwent I/R by the same method while taking levosimendan intraperitoneal 12 µg kg−1; and the diabetic control group (DC) which underwent sham operations without tightening of the coronary sutures. As a control group (C), six healthy age-matched Wistar Albino rats underwent sham operations similar to the DC group. Two hours after the operation, the rats were sacrificed and the myocardial tissue samples were examined by light microscopy for evidence of myonecrosis and inflammatory cell infiltration. Results: Myonecrosis findings were significantly different among groups (p=0.008). Myonecrosis was more pronounced in the DIR group compared with the C, DC, and DIRL groups (p=0.001, p=0.007 and p=0.037, respectively). Similarly, the degree of inflammatory cell infiltration showed significant difference among groups (p<0.0001). Compared with C, DC, and DIRL groups, the inflammatory cell infiltration was significantly higher among the DIR group (p<0.0001, p<0.0001, and p=0.020, respectively). Also, myocardial tissue edema was significantly different among groups (p=0.006). The light microscopic myocardial tissue edema levels were significantly higher in the DIR group than the C, DC, and DIRL groups (p=0.001, p=0.037, and p=0.014, respectively). Conclusion: Taken together, our data indicate that levosimendan may be helpful in reducing myocardial necrosis, myocardial inflammation, and myocardial tissue edema resulting from ischemia–reperfusion injury.http://www.libyanjournalofmedicine.net/index.php/ljm/article/view/29269/pdf_46ischemia–reperfusionlevosimendanmyonecrosisinflammatory cell infiltrationdiabetic rat |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hasan Ali Kiraz Fatih Poyraz Gülay Kip Özlem Erdem Metin Alkan Mustafa Arslan Abdullah Özer Volkan Şivgin Faruk Metin Çomu |
spellingShingle |
Hasan Ali Kiraz Fatih Poyraz Gülay Kip Özlem Erdem Metin Alkan Mustafa Arslan Abdullah Özer Volkan Şivgin Faruk Metin Çomu The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats Libyan Journal of Medicine ischemia–reperfusion levosimendan myonecrosis inflammatory cell infiltration diabetic rat |
author_facet |
Hasan Ali Kiraz Fatih Poyraz Gülay Kip Özlem Erdem Metin Alkan Mustafa Arslan Abdullah Özer Volkan Şivgin Faruk Metin Çomu |
author_sort |
Hasan Ali Kiraz |
title |
The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats |
title_short |
The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats |
title_full |
The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats |
title_fullStr |
The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats |
title_full_unstemmed |
The effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats |
title_sort |
effect of levosimendan on myocardial ischemia–reperfusion injury in streptozotocin-induced diabetic rats |
publisher |
Taylor & Francis Group |
series |
Libyan Journal of Medicine |
issn |
1819-6357 |
publishDate |
2015-12-01 |
description |
Objective: Ischemia/reperfusion (I/R) injury is an important cause of myocardial damage by means of oxidative, inflammatory, and apoptotic mechanisms. The aim of the present study was to examine the potential cardio protective effects of levosimendan in a diabetic rat model of myocardial I/R injury. Methods: A total of 18 streptozotocin-induced diabetic Wistar Albino rats (55 mg/kg) were randomly divided into three equal groups as follows: the diabetic I/R group (DIR) in which myocardial I/R was induced following left thoracotomy, by ligating the left anterior descending coronary artery for 60 min, followed by 2 h of reperfusion; the diabetic I/R levosimendan group (DIRL), which underwent I/R by the same method while taking levosimendan intraperitoneal 12 µg kg−1; and the diabetic control group (DC) which underwent sham operations without tightening of the coronary sutures. As a control group (C), six healthy age-matched Wistar Albino rats underwent sham operations similar to the DC group. Two hours after the operation, the rats were sacrificed and the myocardial tissue samples were examined by light microscopy for evidence of myonecrosis and inflammatory cell infiltration. Results: Myonecrosis findings were significantly different among groups (p=0.008). Myonecrosis was more pronounced in the DIR group compared with the C, DC, and DIRL groups (p=0.001, p=0.007 and p=0.037, respectively). Similarly, the degree of inflammatory cell infiltration showed significant difference among groups (p<0.0001). Compared with C, DC, and DIRL groups, the inflammatory cell infiltration was significantly higher among the DIR group (p<0.0001, p<0.0001, and p=0.020, respectively). Also, myocardial tissue edema was significantly different among groups (p=0.006). The light microscopic myocardial tissue edema levels were significantly higher in the DIR group than the C, DC, and DIRL groups (p=0.001, p=0.037, and p=0.014, respectively). Conclusion: Taken together, our data indicate that levosimendan may be helpful in reducing myocardial necrosis, myocardial inflammation, and myocardial tissue edema resulting from ischemia–reperfusion injury. |
topic |
ischemia–reperfusion levosimendan myonecrosis inflammatory cell infiltration diabetic rat |
url |
http://www.libyanjournalofmedicine.net/index.php/ljm/article/view/29269/pdf_46 |
work_keys_str_mv |
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