Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens
Abstract Background Apicomplexan protozoans of the genus Eimeria cause coccidiosis, one of the most economically relevant parasitic diseases in chickens. The lack of a complete understanding of molecular mechanisms in the host-parasite interaction limits the development of effective control measures...
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doaj-5392117f4cc242e3b4b0ede1217282652020-11-25T03:31:23ZengBMCParasites & Vectors1756-33052019-06-011211910.1186/s13071-019-3534-4Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickensCharles Li0Xianghe Yan1Hyun S. Lillehoj2Sungtaek Oh3Liheng Liu4Zhifeng Sun5Changqin Gu6Youngsub Lee7Zhezi Xianyu8Hongyan Zhao9Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureEnvironmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service-US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of AgricultureAbstract Background Apicomplexan protozoans of the genus Eimeria cause coccidiosis, one of the most economically relevant parasitic diseases in chickens. The lack of a complete understanding of molecular mechanisms in the host-parasite interaction limits the development of effective control measures. In the present study, RNA sequencing (RNA-Seq) was applied to investigate the host mRNA profiles of the cecal mucosa collected at day 5 post-infection with Eimeria maxima (EM). Results Total RNA from cecal samples of the uninfected naïve control and the EM groups was used to make libraries, generating 354,924,372 and 356,229,250 usable reads, respectively, which were assembled into a total of 386,088 high-quality unigenes (transcripts) in Trinity software. RNA-Seq analysis of cecal samples in the two groups revealed 332 upregulated and 363 downregulated genes with significant differences (P ≤ 0.05), including several significant immune-related gene families, such as the major histocompatibility complex (MHC) class I alpha chain, granzyme A and immunoglobulin subtype genes among upregulated differentially expressed genes. In addition, a total of 60 clusters of differentiation (CD) molecular genes and 570 novel genes were found. The completeness of the assembled transcriptome was further assessed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, Gene ontology (GO), eggNOG and CAZy for gene annotation. The broad gene categories represented by the highly differentiated host genes suggested enrichment in immune responses, and downregulation in the metabolic pathway, MARK signaling pathway, vascular smooth muscle contraction, and proteins processing in endoplasmic reticulum after EM infection. Conclusions Eimeria maxima induced statistically significant differences in the cecal mucosal gene expression of infected chickens. These findings provide new insights into the host-parasite interaction and enhance our understanding of the molecular mechanism of avian coccidiosis.http://link.springer.com/article/10.1186/s13071-019-3534-4Eimeria maximaHostCecaRNA-sequencingChickenTranscriptome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Charles Li Xianghe Yan Hyun S. Lillehoj Sungtaek Oh Liheng Liu Zhifeng Sun Changqin Gu Youngsub Lee Zhezi Xianyu Hongyan Zhao |
spellingShingle |
Charles Li Xianghe Yan Hyun S. Lillehoj Sungtaek Oh Liheng Liu Zhifeng Sun Changqin Gu Youngsub Lee Zhezi Xianyu Hongyan Zhao Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens Parasites & Vectors Eimeria maxima Host Ceca RNA-sequencing Chicken Transcriptome |
author_facet |
Charles Li Xianghe Yan Hyun S. Lillehoj Sungtaek Oh Liheng Liu Zhifeng Sun Changqin Gu Youngsub Lee Zhezi Xianyu Hongyan Zhao |
author_sort |
Charles Li |
title |
Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens |
title_short |
Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens |
title_full |
Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens |
title_fullStr |
Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens |
title_full_unstemmed |
Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens |
title_sort |
eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2019-06-01 |
description |
Abstract Background Apicomplexan protozoans of the genus Eimeria cause coccidiosis, one of the most economically relevant parasitic diseases in chickens. The lack of a complete understanding of molecular mechanisms in the host-parasite interaction limits the development of effective control measures. In the present study, RNA sequencing (RNA-Seq) was applied to investigate the host mRNA profiles of the cecal mucosa collected at day 5 post-infection with Eimeria maxima (EM). Results Total RNA from cecal samples of the uninfected naïve control and the EM groups was used to make libraries, generating 354,924,372 and 356,229,250 usable reads, respectively, which were assembled into a total of 386,088 high-quality unigenes (transcripts) in Trinity software. RNA-Seq analysis of cecal samples in the two groups revealed 332 upregulated and 363 downregulated genes with significant differences (P ≤ 0.05), including several significant immune-related gene families, such as the major histocompatibility complex (MHC) class I alpha chain, granzyme A and immunoglobulin subtype genes among upregulated differentially expressed genes. In addition, a total of 60 clusters of differentiation (CD) molecular genes and 570 novel genes were found. The completeness of the assembled transcriptome was further assessed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, Gene ontology (GO), eggNOG and CAZy for gene annotation. The broad gene categories represented by the highly differentiated host genes suggested enrichment in immune responses, and downregulation in the metabolic pathway, MARK signaling pathway, vascular smooth muscle contraction, and proteins processing in endoplasmic reticulum after EM infection. Conclusions Eimeria maxima induced statistically significant differences in the cecal mucosal gene expression of infected chickens. These findings provide new insights into the host-parasite interaction and enhance our understanding of the molecular mechanism of avian coccidiosis. |
topic |
Eimeria maxima Host Ceca RNA-sequencing Chicken Transcriptome |
url |
http://link.springer.com/article/10.1186/s13071-019-3534-4 |
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