Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometers
The efficacy of cellular signal transduction is of paramount importance for the proper functioning of a cell and an organism as a whole. Protein kinases are responsible for much of this transmission and thus have been the focal point of extensive research. While there are numerous commercially avail...
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doaj-539eb6dac8a341c4a9c992b1161b92cd2020-11-25T02:29:30ZengElsevierMethodsX2215-01612014-01-011C9610110.1016/j.mex.2014.07.003Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometersRyan A.V. BellKenneth B. StoreyThe efficacy of cellular signal transduction is of paramount importance for the proper functioning of a cell and an organism as a whole. Protein kinases are responsible for much of this transmission and thus have been the focal point of extensive research. While there are numerous commercially available protein kinase assays, the Kinase-Glo luminescent kinase assay (Promega) provides an easy-to-use and high throughput platform for determining protein kinase activity. This assay is said to require the use of a microplate spectrophotometer capable of detecting a luminescent signal. This study shows that: • The ChemiGenius Bioimaging system (Syngene), typically used for visualizing chemiluminescence from Western blots, provides an alternative detection system for Kinase-Glo luminescence. • The novel detection system confers an advantage over traditional luminometers, in that it allows visualization of the luminescent wells, which allows for the real-time analysis and correction of experimental errors (i.e. bubble formation). • Determining kinase kinetics using this detection system produced comparable results to previous studies on the same enzyme (i.e. glycogen synthase kinase 3).http://www.sciencedirect.com/science/article/pii/S2215016114200604Protein kinase assayKinase-GloMicroplate luminescence detection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ryan A.V. Bell Kenneth B. Storey |
spellingShingle |
Ryan A.V. Bell Kenneth B. Storey Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometers MethodsX Protein kinase assay Kinase-Glo Microplate luminescence detection |
author_facet |
Ryan A.V. Bell Kenneth B. Storey |
author_sort |
Ryan A.V. Bell |
title |
Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometers |
title_short |
Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometers |
title_full |
Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometers |
title_fullStr |
Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometers |
title_full_unstemmed |
Novel detection method for chemiluminescence derived from the Kinase-Glo luminescent kinase assay platform: Advantages over traditional microplate luminometers |
title_sort |
novel detection method for chemiluminescence derived from the kinase-glo luminescent kinase assay platform: advantages over traditional microplate luminometers |
publisher |
Elsevier |
series |
MethodsX |
issn |
2215-0161 |
publishDate |
2014-01-01 |
description |
The efficacy of cellular signal transduction is of paramount importance for the proper functioning of a cell and an organism as a whole. Protein kinases are responsible for much of this transmission and thus have been the focal point of extensive research. While there are numerous commercially available protein kinase assays, the Kinase-Glo luminescent kinase assay (Promega) provides an easy-to-use and high throughput platform for determining protein kinase activity. This assay is said to require the use of a microplate spectrophotometer capable of detecting a luminescent signal. This study shows that:
•
The ChemiGenius Bioimaging system (Syngene), typically used for visualizing chemiluminescence from Western blots, provides an alternative detection system for Kinase-Glo luminescence.
•
The novel detection system confers an advantage over traditional luminometers, in that it allows visualization of the luminescent wells, which allows for the real-time analysis and correction of experimental errors (i.e. bubble formation).
•
Determining kinase kinetics using this detection system produced comparable results to previous studies on the same enzyme (i.e. glycogen synthase kinase 3). |
topic |
Protein kinase assay Kinase-Glo Microplate luminescence detection |
url |
http://www.sciencedirect.com/science/article/pii/S2215016114200604 |
work_keys_str_mv |
AT ryanavbell noveldetectionmethodforchemiluminescencederivedfromthekinasegloluminescentkinaseassayplatformadvantagesovertraditionalmicroplateluminometers AT kennethbstorey noveldetectionmethodforchemiluminescencederivedfromthekinasegloluminescentkinaseassayplatformadvantagesovertraditionalmicroplateluminometers |
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