Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography
Rodents are popular biological models for physiological and behavioral research in neuroscience and rats are better models than mice due to their higher genome similarity to human and more accessible surgical procedures. However, rat brain is larger than mice brain and it needs powerful imaging tool...
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2019-11-01
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doaj-53a08ca5b2ca42778cabdedad647b62c2020-11-25T02:09:30ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052019-11-011261950012-11950012-1210.1142/S179354581950012310.1142/S1793545819500123Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiographyHequn Zhang0Weisi Xie1Ming Chen2Liang Zhu3Zhe Feng4Yalun Wang5Wang Xi6Ben Zhong Tang7Jun Qian8Centre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou 310058, P. R. ChinaCentre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou 310058, P. R. ChinaDepartment of Chemistry Hong Kong Branch of Chinese National Engineering, Research Center for Tissue Restoration and Reconstruction, Division of Life Science, State Key Laboratory of Molecular Neuroscience, Institute for Advanced Study, Institute of Molecular Functional Materials, Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P. R. ChinaInterdisciplinary Institute of Neuroscience and Technology (ZIINT), Zhejiang University, Hangzhou, 310058, P. R. ChinaCentre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou 310058, P. R. ChinaCentre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou 310058, P. R. ChinaInterdisciplinary Institute of Neuroscience and Technology (ZIINT), Zhejiang University, Hangzhou, 310058, P. R. ChinaDepartment of Chemistry Hong Kong Branch of Chinese National Engineering, Research Center for Tissue Restoration and Reconstruction, Division of Life Science, State Key Laboratory of Molecular Neuroscience, Institute for Advanced Study, Institute of Molecular Functional Materials, Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, P. R. ChinaCentre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou 310058, P. R. ChinaRodents are popular biological models for physiological and behavioral research in neuroscience and rats are better models than mice due to their higher genome similarity to human and more accessible surgical procedures. However, rat brain is larger than mice brain and it needs powerful imaging tools to implement better penetration against the scattering of the thicker brain tissue. Three-photon fluorescence microscopy (3PFM) combined with near-infrared (NIR) excitation has great potentials for brain circuits imaging because of its abilities of anti-scattering, deep-tissue imaging, and high signal-to-noise ratio (SNR). In this work, a type of AIE luminogen with red fluorescence was synthesized and encapsulated with Pluronic F-127 to make up form nanoparticles (NPs). Bright DCDPP-2TPA NPs were employed for in vivo three-photon fluorescent laser scanning microscopy of blood vessels in rats brain under 1550nm femtosecond laser excitation. A fine three-dimensional (3D) reconstruction up to the deepness of 600μm was achieved and the blood flow velocity of a selected vessel was measured in vivo as well. Our 3PFM deep brain imaging method simultaneously recorded the morphology and function of the brain blood vessels in vivo in the rat model. Using this angiography combined with the arsenal of rodent’s brain disease, models can accelerate the neuroscience research and clinical diagnosis of brain disease in the future.http://www.worldscientific.com/doi/pdf/10.1142/S1793545819500123three-photon fluorescence microscopy (3pfm)aggregation-induced emission (aie)deep-tissue imagingin vivorat brain |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hequn Zhang Weisi Xie Ming Chen Liang Zhu Zhe Feng Yalun Wang Wang Xi Ben Zhong Tang Jun Qian |
spellingShingle |
Hequn Zhang Weisi Xie Ming Chen Liang Zhu Zhe Feng Yalun Wang Wang Xi Ben Zhong Tang Jun Qian Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography Journal of Innovative Optical Health Sciences three-photon fluorescence microscopy (3pfm) aggregation-induced emission (aie) deep-tissue imaging in vivo rat brain |
author_facet |
Hequn Zhang Weisi Xie Ming Chen Liang Zhu Zhe Feng Yalun Wang Wang Xi Ben Zhong Tang Jun Qian |
author_sort |
Hequn Zhang |
title |
Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography |
title_short |
Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography |
title_full |
Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography |
title_fullStr |
Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography |
title_full_unstemmed |
Aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography |
title_sort |
aggregation-induced emission nanoparticles for in vivo three-photon fluorescence microscopic rat brain angiography |
publisher |
World Scientific Publishing |
series |
Journal of Innovative Optical Health Sciences |
issn |
1793-5458 1793-7205 |
publishDate |
2019-11-01 |
description |
Rodents are popular biological models for physiological and behavioral research in neuroscience and rats are better models than mice due to their higher genome similarity to human and more accessible surgical procedures. However, rat brain is larger than mice brain and it needs powerful imaging tools to implement better penetration against the scattering of the thicker brain tissue. Three-photon fluorescence microscopy (3PFM) combined with near-infrared (NIR) excitation has great potentials for brain circuits imaging because of its abilities of anti-scattering, deep-tissue imaging, and high signal-to-noise ratio (SNR). In this work, a type of AIE luminogen with red fluorescence was synthesized and encapsulated with Pluronic F-127 to make up form nanoparticles (NPs). Bright DCDPP-2TPA NPs were employed for in vivo three-photon fluorescent laser scanning microscopy of blood vessels in rats brain under 1550nm femtosecond laser excitation. A fine three-dimensional (3D) reconstruction up to the deepness of 600μm was achieved and the blood flow velocity of a selected vessel was measured in vivo as well. Our 3PFM deep brain imaging method simultaneously recorded the morphology and function of the brain blood vessels in vivo in the rat model. Using this angiography combined with the arsenal of rodent’s brain disease, models can accelerate the neuroscience research and clinical diagnosis of brain disease in the future. |
topic |
three-photon fluorescence microscopy (3pfm) aggregation-induced emission (aie) deep-tissue imaging in vivo rat brain |
url |
http://www.worldscientific.com/doi/pdf/10.1142/S1793545819500123 |
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