Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro
Biomaterials that provide three-dimensional support networks for the culture of cells are being developed for a wide range of tissue engineering applications including the regeneration of bone. This study explores the potential of the versatile ionic-complementary peptide, FEFEFKFK, for such a purpo...
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2014-07-01
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Series: | Journal of Tissue Engineering |
Online Access: | https://doi.org/10.1177/2041731414539344 |
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doaj-53b41dfe924a4a159676f6094d29f8922020-11-25T02:50:41ZengSAGE PublishingJournal of Tissue Engineering2041-73142014-07-01510.1177/204173141453934410.1177_2041731414539344Human osteoblasts within soft peptide hydrogels promote mineralisation in vitroLuis A Castillo Diaz0Alberto Saiani1Julie E Gough2Aline F Miller3Manchester Institute of Biotechnology, The University of Manchester, Manchester, UKSchool of Materials, The University of Manchester, Manchester, UKSchool of Materials, The University of Manchester, Manchester, UKManchester Institute of Biotechnology, The University of Manchester, Manchester, UKBiomaterials that provide three-dimensional support networks for the culture of cells are being developed for a wide range of tissue engineering applications including the regeneration of bone. This study explores the potential of the versatile ionic-complementary peptide, FEFEFKFK, for such a purpose as this peptide spontaneously self-assembles into β-sheet-rich fibres that subsequently self-associate to form self-supporting hydrogels. Via simple live/dead cell assays, we demonstrated that 3 wt% hydrogels were optimal for the support of osteoblast cells. We went on to show that these cells are not only viable within the three-dimensional hydrogel but they also proliferate and produce osteogenic key proteins, that is, they behave like in vivo bone cells, over the 14-day period explored here. The gel elasticity increased over time when cells were present – in comparison to a decrease in control samples – indicating the deposition of matrix throughout the peptide scaffold. Moreover, significant quantities of calcium phosphate were deposited. Collectively, these data demonstrate that ionic-complementary octapeptides offer a suitable three-dimensional environment for osteoblastic cell function.https://doi.org/10.1177/2041731414539344 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luis A Castillo Diaz Alberto Saiani Julie E Gough Aline F Miller |
spellingShingle |
Luis A Castillo Diaz Alberto Saiani Julie E Gough Aline F Miller Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro Journal of Tissue Engineering |
author_facet |
Luis A Castillo Diaz Alberto Saiani Julie E Gough Aline F Miller |
author_sort |
Luis A Castillo Diaz |
title |
Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro |
title_short |
Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro |
title_full |
Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro |
title_fullStr |
Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro |
title_full_unstemmed |
Human osteoblasts within soft peptide hydrogels promote mineralisation in vitro |
title_sort |
human osteoblasts within soft peptide hydrogels promote mineralisation in vitro |
publisher |
SAGE Publishing |
series |
Journal of Tissue Engineering |
issn |
2041-7314 |
publishDate |
2014-07-01 |
description |
Biomaterials that provide three-dimensional support networks for the culture of cells are being developed for a wide range of tissue engineering applications including the regeneration of bone. This study explores the potential of the versatile ionic-complementary peptide, FEFEFKFK, for such a purpose as this peptide spontaneously self-assembles into β-sheet-rich fibres that subsequently self-associate to form self-supporting hydrogels. Via simple live/dead cell assays, we demonstrated that 3 wt% hydrogels were optimal for the support of osteoblast cells. We went on to show that these cells are not only viable within the three-dimensional hydrogel but they also proliferate and produce osteogenic key proteins, that is, they behave like in vivo bone cells, over the 14-day period explored here. The gel elasticity increased over time when cells were present – in comparison to a decrease in control samples – indicating the deposition of matrix throughout the peptide scaffold. Moreover, significant quantities of calcium phosphate were deposited. Collectively, these data demonstrate that ionic-complementary octapeptides offer a suitable three-dimensional environment for osteoblastic cell function. |
url |
https://doi.org/10.1177/2041731414539344 |
work_keys_str_mv |
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