Sialylation and muscle performance: sialic acid is a marker of muscle ageing.
Sialic acids (Sia) are widely expressed as terminal monosaccharides on eukaryotic glycoconjugates. They are involved in many cellular functions, such as cell-cell interaction and signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-...
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doaj-53bd6e0d6e864e77a2ddbf7051eced7b2021-03-03T20:18:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8052010.1371/journal.pone.0080520Sialylation and muscle performance: sialic acid is a marker of muscle ageing.Frank HanischWenke WeidemannMona GroßmannPushpa Raj JoshiHans-Jürgen HolzhausenGisela StoltenburgJoachim WeisStephan ZierzRüdiger HorstkorteSialic acids (Sia) are widely expressed as terminal monosaccharides on eukaryotic glycoconjugates. They are involved in many cellular functions, such as cell-cell interaction and signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyses the first two steps of Sia biosynthesis in the cytosol. In this study we analysed sialylation of muscles in wild type (C57Bl/6 GNE (+/+)) and heterozygous GNE-deficient (C57Bl/6 GNE (+/-)) mice. We measured a significantly lower performance in the initial weeks of a treadmill exercise in C57Bl/6 GNE (+/-) mice compared to wild type C57Bl/6 GNE (+/+) animals. Membrane bound Sia of C57Bl/6 GNE (+/-) mice were reduced by 33-53% at week 24 and by 12-15% at week 80 in comparison to C57Bl/6 GNE (+/+) mice. Interestingly, membrane bound Sia concentration increased with age of the mice by 16-46% in C57Bl/6 GNE (+/+), but by 87-207% in C57Bl/6 GNE (+/-). Furthermore we could identify specific morphological changes in aged muscles. Here we propose that increased Sia concentrations in muscles are a characteristic feature of ageing and could be used as a marker for age-related changes in muscle.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349002/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Frank Hanisch Wenke Weidemann Mona Großmann Pushpa Raj Joshi Hans-Jürgen Holzhausen Gisela Stoltenburg Joachim Weis Stephan Zierz Rüdiger Horstkorte |
spellingShingle |
Frank Hanisch Wenke Weidemann Mona Großmann Pushpa Raj Joshi Hans-Jürgen Holzhausen Gisela Stoltenburg Joachim Weis Stephan Zierz Rüdiger Horstkorte Sialylation and muscle performance: sialic acid is a marker of muscle ageing. PLoS ONE |
author_facet |
Frank Hanisch Wenke Weidemann Mona Großmann Pushpa Raj Joshi Hans-Jürgen Holzhausen Gisela Stoltenburg Joachim Weis Stephan Zierz Rüdiger Horstkorte |
author_sort |
Frank Hanisch |
title |
Sialylation and muscle performance: sialic acid is a marker of muscle ageing. |
title_short |
Sialylation and muscle performance: sialic acid is a marker of muscle ageing. |
title_full |
Sialylation and muscle performance: sialic acid is a marker of muscle ageing. |
title_fullStr |
Sialylation and muscle performance: sialic acid is a marker of muscle ageing. |
title_full_unstemmed |
Sialylation and muscle performance: sialic acid is a marker of muscle ageing. |
title_sort |
sialylation and muscle performance: sialic acid is a marker of muscle ageing. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Sialic acids (Sia) are widely expressed as terminal monosaccharides on eukaryotic glycoconjugates. They are involved in many cellular functions, such as cell-cell interaction and signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyses the first two steps of Sia biosynthesis in the cytosol. In this study we analysed sialylation of muscles in wild type (C57Bl/6 GNE (+/+)) and heterozygous GNE-deficient (C57Bl/6 GNE (+/-)) mice. We measured a significantly lower performance in the initial weeks of a treadmill exercise in C57Bl/6 GNE (+/-) mice compared to wild type C57Bl/6 GNE (+/+) animals. Membrane bound Sia of C57Bl/6 GNE (+/-) mice were reduced by 33-53% at week 24 and by 12-15% at week 80 in comparison to C57Bl/6 GNE (+/+) mice. Interestingly, membrane bound Sia concentration increased with age of the mice by 16-46% in C57Bl/6 GNE (+/+), but by 87-207% in C57Bl/6 GNE (+/-). Furthermore we could identify specific morphological changes in aged muscles. Here we propose that increased Sia concentrations in muscles are a characteristic feature of ageing and could be used as a marker for age-related changes in muscle. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349002/?tool=EBI |
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