Time Needed for Rinsing the Decalcification Specimen by Tap Water
Objectives : To determine a proper time for neutralizing the decalcified specimen by rinsing with tap water. Methods : Fix pork from fresh market in routine fixative for 24 hours, put into 5% nitric acid for 24 hours to 7 days. Then rinse in running tap water for 1-48 hours. Process and cut the spe...
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Mahidol University
2003-07-01
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Online Access: | https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245417 |
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doaj-53c352948d194e08a102dd3521243d442021-08-13T10:07:14ZengMahidol UniversitySiriraj Medical Journal2228-80822003-07-01557Time Needed for Rinsing the Decalcification Specimen by Tap WaterMongkol Uiprasertkul0Soranart Muangsomboon1Ananya Manuyakorn2Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol UniversityDepartment of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol UniversityDepartment of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University Objectives : To determine a proper time for neutralizing the decalcified specimen by rinsing with tap water. Methods : Fix pork from fresh market in routine fixative for 24 hours, put into 5% nitric acid for 24 hours to 7 days. Then rinse in running tap water for 1-48 hours. Process and cut the specimens into slide, attain with H&E stain. Distribute to three pathologists to evaluate the quality of staining and microscopic detail. Results : Neutralizing for 2 hours or longer yields similar microscopic to the specimen that soaked in acid for 72 hours or less. The histologic feature is not recovered in the soaked in acid longer than 72 hours, no matter how long it is neutralized. Discussion : The result shows no benefit in rinsing the decalcified specimen longer than 2 hours, and soaking in acid longer than 72 hours causes permanent artifact. https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245417Decalcificationrinseneutralizing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mongkol Uiprasertkul Soranart Muangsomboon Ananya Manuyakorn |
spellingShingle |
Mongkol Uiprasertkul Soranart Muangsomboon Ananya Manuyakorn Time Needed for Rinsing the Decalcification Specimen by Tap Water Siriraj Medical Journal Decalcification rinse neutralizing |
author_facet |
Mongkol Uiprasertkul Soranart Muangsomboon Ananya Manuyakorn |
author_sort |
Mongkol Uiprasertkul |
title |
Time Needed for Rinsing the Decalcification Specimen by Tap Water |
title_short |
Time Needed for Rinsing the Decalcification Specimen by Tap Water |
title_full |
Time Needed for Rinsing the Decalcification Specimen by Tap Water |
title_fullStr |
Time Needed for Rinsing the Decalcification Specimen by Tap Water |
title_full_unstemmed |
Time Needed for Rinsing the Decalcification Specimen by Tap Water |
title_sort |
time needed for rinsing the decalcification specimen by tap water |
publisher |
Mahidol University |
series |
Siriraj Medical Journal |
issn |
2228-8082 |
publishDate |
2003-07-01 |
description |
Objectives : To determine a proper time for neutralizing the decalcified specimen by rinsing with tap water.
Methods : Fix pork from fresh market in routine fixative for 24 hours, put into 5% nitric acid for 24 hours to 7 days. Then rinse in running tap water for 1-48 hours. Process and cut the specimens into slide, attain with H&E stain. Distribute to three pathologists to evaluate the quality of staining and microscopic detail.
Results : Neutralizing for 2 hours or longer yields similar microscopic to the specimen that soaked in acid for 72 hours or less. The histologic feature is not recovered in the soaked in acid longer than 72 hours, no matter how long it is neutralized.
Discussion : The result shows no benefit in rinsing the decalcified specimen longer than 2 hours, and soaking in acid longer than 72 hours causes permanent artifact.
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topic |
Decalcification rinse neutralizing |
url |
https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245417 |
work_keys_str_mv |
AT mongkoluiprasertkul timeneededforrinsingthedecalcificationspecimenbytapwater AT soranartmuangsomboon timeneededforrinsingthedecalcificationspecimenbytapwater AT ananyamanuyakorn timeneededforrinsingthedecalcificationspecimenbytapwater |
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