Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved]
Background: Studying astrocytes in higher brain functions has been hampered by the lack of genetic tools for the efficient expression of inducible Cre recombinase throughout the CNS, including the neocortex. Methods: Therefore, we generated BAC transgenic mice, in which CreERT2 is expressed under co...
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doaj-53c8f73488c14ec299224e06b6b62fe42020-11-25T03:49:51ZengF1000 Research LtdF1000Research2046-14022016-12-01510.12688/f1000research.10509.111327Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved]Jan Winchenbach0Tim Düking1Stefan A. Berghoff2Sina K. Stumpf3Swen Hülsmann4Klaus-Armin Nave5Gesine Saher6Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, GermanyDepartment of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, GermanyDepartment of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, GermanyDepartment of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, GermanyCenter Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, GermanyCenter Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, GermanyDepartment of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, GermanyBackground: Studying astrocytes in higher brain functions has been hampered by the lack of genetic tools for the efficient expression of inducible Cre recombinase throughout the CNS, including the neocortex. Methods: Therefore, we generated BAC transgenic mice, in which CreERT2 is expressed under control of the Aldh1l1 regulatory region. Results: When crossbred to Cre reporter mice, adult Aldh1l1-CreERT2 mice show efficient gene targeting in astrocytes. No such Cre-mediated recombination was detectable in CNS neurons, oligodendrocytes, and microglia. As expected, Aldh1l1-CreERT2 expression was evident in several peripheral organs, including liver and kidney. Conclusions: Taken together, Aldh1l1-CreERT2 mice are a useful tool for studying astrocytes in neurovascular coupling, brain metabolism, synaptic plasticity and other aspects of neuron-glia interactions.https://f1000research.com/articles/5-2934/v1Neurogenetics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan Winchenbach Tim Düking Stefan A. Berghoff Sina K. Stumpf Swen Hülsmann Klaus-Armin Nave Gesine Saher |
spellingShingle |
Jan Winchenbach Tim Düking Stefan A. Berghoff Sina K. Stumpf Swen Hülsmann Klaus-Armin Nave Gesine Saher Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved] F1000Research Neurogenetics |
author_facet |
Jan Winchenbach Tim Düking Stefan A. Berghoff Sina K. Stumpf Swen Hülsmann Klaus-Armin Nave Gesine Saher |
author_sort |
Jan Winchenbach |
title |
Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved] |
title_short |
Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved] |
title_full |
Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved] |
title_fullStr |
Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved] |
title_full_unstemmed |
Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice [version 1; referees: 2 approved] |
title_sort |
inducible targeting of cns astrocytes in aldh1l1-creert2 bac transgenic mice [version 1; referees: 2 approved] |
publisher |
F1000 Research Ltd |
series |
F1000Research |
issn |
2046-1402 |
publishDate |
2016-12-01 |
description |
Background: Studying astrocytes in higher brain functions has been hampered by the lack of genetic tools for the efficient expression of inducible Cre recombinase throughout the CNS, including the neocortex. Methods: Therefore, we generated BAC transgenic mice, in which CreERT2 is expressed under control of the Aldh1l1 regulatory region. Results: When crossbred to Cre reporter mice, adult Aldh1l1-CreERT2 mice show efficient gene targeting in astrocytes. No such Cre-mediated recombination was detectable in CNS neurons, oligodendrocytes, and microglia. As expected, Aldh1l1-CreERT2 expression was evident in several peripheral organs, including liver and kidney. Conclusions: Taken together, Aldh1l1-CreERT2 mice are a useful tool for studying astrocytes in neurovascular coupling, brain metabolism, synaptic plasticity and other aspects of neuron-glia interactions. |
topic |
Neurogenetics |
url |
https://f1000research.com/articles/5-2934/v1 |
work_keys_str_mv |
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