Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats
Background: We assessed the effect of acute and chronic dietary supplementation of ω-3 on lipid metabolism and cardiac regeneration, through its influence on the Stromal Derived Factor-1 (SDF-1) and its receptor (CXCR4) axis in normotensive and hypertensive rats. Methods: Male Wistar Kyoto (WKY) and...
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doaj-53cf85a74ebf4915bf859c4f374437b32020-11-24T21:27:50ZengMDPI AGNutrients2072-66432017-08-019882610.3390/nu9080826nu9080826Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive RatsLuiza Halmenschlager0Alexandre Machado Lehnen1Aline Marcadenti2Melissa Medeiros Markoski3Postgraduate Program in Health Sciences: Cardiology, Institute of Cardiology of Rio Grande do Sul/University Foundation of Cardiology (IC/FUC), Princesa Isabel Avenue, 370, Porto Alegre, RS 90620-001, BrazilPostgraduate Program in Health Sciences: Cardiology, Institute of Cardiology of Rio Grande do Sul/University Foundation of Cardiology (IC/FUC), Princesa Isabel Avenue, 370, Porto Alegre, RS 90620-001, BrazilPostgraduate Program in Health Sciences: Cardiology, Institute of Cardiology of Rio Grande do Sul/University Foundation of Cardiology (IC/FUC), Princesa Isabel Avenue, 370, Porto Alegre, RS 90620-001, BrazilPostgraduate Program in Health Sciences: Cardiology, Institute of Cardiology of Rio Grande do Sul/University Foundation of Cardiology (IC/FUC), Princesa Isabel Avenue, 370, Porto Alegre, RS 90620-001, BrazilBackground: We assessed the effect of acute and chronic dietary supplementation of ω-3 on lipid metabolism and cardiac regeneration, through its influence on the Stromal Derived Factor-1 (SDF-1) and its receptor (CXCR4) axis in normotensive and hypertensive rats. Methods: Male Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were allocated in eight groups (of eight animals each), which received daily orogastric administration of ω-3 (1 g) for 24 h, 72 h or 2 weeks. Blood samples were collected for the analysis of the lipid profile and SDF-1 systemic levels (ELISA). At the end of the treatment period, cardiac tissue was collected for CXCR4 expression analysis (Western blot). Results: The use of ω-3 caused a reduction in total cholesterol levels (p = 0.044), and acutely activated the SDF-1/CXCR4 axis in normotensive animals (p = 0.037). In the presence of the ω-3, after 72 h, SDF-1 levels decreased in WKY and increased in SHR (p = 0.017), and tissue expression of the receptor CXCR4 was higher in WKY than in SHR (p = 0.001). Conclusion: The ω-3 fatty acid supplementation differentially modulates cell homing mediators in normotensive and hypertensive animals. While WKY rats respond acutely to omega-3 supplementation, showing increased release of SDF-1 and CXCR4, SHR exhibit a weaker, delayed response.https://www.mdpi.com/2072-6643/9/8/826ω-3 fatty acidspontaneously hypertensive ratsWistar Kyoto ratscell hominghypertensionStromal Derived Factor-1CXCR4 receptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luiza Halmenschlager Alexandre Machado Lehnen Aline Marcadenti Melissa Medeiros Markoski |
spellingShingle |
Luiza Halmenschlager Alexandre Machado Lehnen Aline Marcadenti Melissa Medeiros Markoski Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats Nutrients ω-3 fatty acid spontaneously hypertensive rats Wistar Kyoto rats cell homing hypertension Stromal Derived Factor-1 CXCR4 receptor |
author_facet |
Luiza Halmenschlager Alexandre Machado Lehnen Aline Marcadenti Melissa Medeiros Markoski |
author_sort |
Luiza Halmenschlager |
title |
Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats |
title_short |
Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats |
title_full |
Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats |
title_fullStr |
Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats |
title_full_unstemmed |
Omega-3 Fatty Acids Supplementation Differentially Modulates the SDF-1/CXCR-4 Cell Homing Axis in Hypertensive and Normotensive Rats |
title_sort |
omega-3 fatty acids supplementation differentially modulates the sdf-1/cxcr-4 cell homing axis in hypertensive and normotensive rats |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2017-08-01 |
description |
Background: We assessed the effect of acute and chronic dietary supplementation of ω-3 on lipid metabolism and cardiac regeneration, through its influence on the Stromal Derived Factor-1 (SDF-1) and its receptor (CXCR4) axis in normotensive and hypertensive rats. Methods: Male Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were allocated in eight groups (of eight animals each), which received daily orogastric administration of ω-3 (1 g) for 24 h, 72 h or 2 weeks. Blood samples were collected for the analysis of the lipid profile and SDF-1 systemic levels (ELISA). At the end of the treatment period, cardiac tissue was collected for CXCR4 expression analysis (Western blot). Results: The use of ω-3 caused a reduction in total cholesterol levels (p = 0.044), and acutely activated the SDF-1/CXCR4 axis in normotensive animals (p = 0.037). In the presence of the ω-3, after 72 h, SDF-1 levels decreased in WKY and increased in SHR (p = 0.017), and tissue expression of the receptor CXCR4 was higher in WKY than in SHR (p = 0.001). Conclusion: The ω-3 fatty acid supplementation differentially modulates cell homing mediators in normotensive and hypertensive animals. While WKY rats respond acutely to omega-3 supplementation, showing increased release of SDF-1 and CXCR4, SHR exhibit a weaker, delayed response. |
topic |
ω-3 fatty acid spontaneously hypertensive rats Wistar Kyoto rats cell homing hypertension Stromal Derived Factor-1 CXCR4 receptor |
url |
https://www.mdpi.com/2072-6643/9/8/826 |
work_keys_str_mv |
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